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Report No.
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Comprehensive detection of radiation-induced bystander effects using co-culture system

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Muto, Yasuko; Kanai, Tatsuaki*; Kobayashi, Yasuhiko

The purpose of this research is to detect comprehensively what kind of influence radiation-induced bystander effects have, between lung normal and cancer cells using co-culture system. In our experiments, human lung normal fibroblasts WI-38 line and human lung cancer cells H1299/wt${it p53}$ line were used. Cells were irradiated with carbon-ion broad beams (WI-38: 0.13 Gy, H1299/wt${it p53}$: 0.5 Gy) or $$^{60}$$Co $$gamma$$-rays (0.5 Gy), then survival rates of bystander cells after 6- or 24-hours co-culture with irradiated cells was calculated using colony formation assay. When we co-cultured irradiated H1299/wt${it p53}$ with non-irradiated WI-38, it was found that survival rates of WI-38 increased about 10% after 24-hours co-culture. On the other hand, when we co-cultured irradiated WI-38 with non-irradiated WI-38, it was found survival rates of WI-38 decreased about 15% after 6-hours and 24-hours co-culture. Consequently, when the kinds of irradiated cells differed, it found out that heavy-ion induced bystander responses change a lot, and it was thought that these results are important to upgrade heavy-ion radiotherapy.

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