Construction of experimental systems to analyze bystander effects induced by heavy-ion microbeam between different type cells

Ikeda, Hiroko; Funayama, Tomoo; Yokota, Yuichiro; Kanai, Tatsuaki*; Kobayashi, Yasuhiko

We have so far analyzed bystander effects between lung cancer cells H1299/wt and lung normal cells WI-38, using clonogenic assay to judge reproductive cell death. Consequently, when the kinds of irradiated cells differed, it found out that heavy-ion induced bystander responses change a lot. In this research, we decided to use the heavy-ion microbeam system at JAEA-Takasaki to clarify inner cellular and/or intercellular molecular mechanisms concerned with the bystander effects. This equipment is an effective tool and it enabled to elucidate mechanisms which were not made clearly in our previous study using broad beam system. Although there are many reports of bystander effects between the same type cells using microbeam, since there is still no report of bystander effects between different type cells using heavy-ion microbeam, the establishment of experimental system is needed. Now, we are constructing experimental systems to irradiate a part of cells which carried out mixed culture in the same plate using microbeam, and we will report the acquired findings and future development.