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Observation of apoptotic nuclei by a laser-plasma soft X-ray microscope

Kado, Masataka; Kishimoto, Maki; Tone, Shigenobu*; Tamotsu, Satoshi*; Yasuda, Keiko*; Aoyama, Masato*; Shinohara, Kunio*

Laser plasma soft X-ray microscope using a laser plasma X-ray source has high spatial resolution of 100 nm and high temporal resolution of 1 nm and has advantage to be able to observe live hydrated biological cells. We have succeeded to observe cellular organelles such as mitochondria, cytoskeleton, and chromatin structures. We also invented to use a fluorescent microscope along with the soft X-ray microscope in order to identify cellular organelles obtained in the soft X-ray images. As the next application for the soft X-ray microscope Apoptotic nuclei have been observed. Since the laser plasma soft X-ray microscope has higher spatial resolution than the fluorescent microscope and can observe the structural change directory, it is expected to accomplish important role to understand apoptotic mechanism.

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