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Report No.

FACS-based screening for yeast clone highly expressing cellulase

Ninomiya, Kazuaki*; Nomura, Tomoyo*; Sato, Katsuya; Narumi, Issei; Shimizu, Nobuaki*

By using immunocytochemistry and fluorescence-activated cell sorter (FACS), yeast population highly expressing cellulase on the cell surface was enriched from the mutant population prepared by irradiation with carbon ion beams (220 MeV $$^{12}$$C$$^{5+}$$, 100 Gy). Enzymatic activity of the enriched clones was quantified in vitro by a conventional biochemical method. Repeated FACS after irradiation with carbon ion beams was effective for selecting yeast population of interest.



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