イオンビーム育種で作出したカプロン酸エチル高生産清酒酵母の変異部位の同定

Identification of mutation sites in high ethyl caproate producing sake yeasts generated by ion beam breeding

増淵 隆*; 高島 智里*; 上山 修*; 池永 裕*; 佐藤 勝也; 鳴海 一成

Masubuchi, Takashi*; Takashima, Chisato*; Kamiyama, Osamu*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issei

In the previous study, we have obtained high ethyl caproate producing Sake yeasts No.227 and No.1333 by carbon-ion irradiation. In this study, to identify the mutation sites of the mutant strains, the specific region (226 bp; nucleotide positions 3,642-3,867) in the fatty acid synthase gene that might be involved in the higher production of ethyl caproate was sequenced and compared with the corresponding sequence in the parental strain Kyokai-901. Consequently, strains No.227 and No.1333 carried G/A and A/C mutations, respectively, at nucleotide position 3,748 as a heterozygous state. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in gene.