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Report No.

Comprehensive detection of radiation-induced bystander effects using lung normal and cancer cells

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Kanai, Tatsuaki*; Kobayashi, Yasuhiko

The purpose of this research is to detect comprehensively what kind of influence radiation-induced bystander effects have, between lung normal cells WI-38 and lung cancer cells H1299/wt${it p53}$ using co-culture system. In our experiments, cells were irradiated with carbon-ion broad beams (WI-38: 0.13 Gy, H1299/wt${it p53}$: 0.5 Gy), then survival rates of bystander cells after 6- or 24-hours co-culture with irradiated cells was calculated using colony formation assay. Consequently, it was thought that survival rates of non-irradiated cell were increased by co-culture between different cells. So we used the heavy-ion microbeam system at JAEA-Takasaki to clarify molecular mechanisms concerned with the bystander effects. Now, we are constructing experimental systems to irradiate a part of cells which carried out mixed culture in the same plate using microbeam, and we will report the acquired findings.



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