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Dependence of the yields of AP sites and AP clusters produced in plasmid DNA on scavenging capacity and LET

放射線によりプラスミドDNA中に生じたAPサイトとこれを含むクラスター損傷の収率のラジカル補足能及びLET依存性

椎名 卓也*; 渡辺 立子; 鈴木 雅雄*; 横谷 明徳

Shiina, Takuya*; Watanabe, Ritsuko; Suzuki, Masao*; Yokoya, Akinari

The object of the study is to reveal whether the induction process of an abasic site (AP site) and the clustered DNA damage which contain AP sites (AP cluster) depends on ionizing density of radiation. In order to clarify the relation between track structure of C ions and the induction processes of an AP site or AP cluster, we measure the yield of AP using Nfo protein as an enzymatic probe, which converts an AP site to detectable single strand break. Several scavenging capacities in the samples are tested to estimate the effect of OH radicals. We compare those experimental yields with theoretical ones that are obtained from Monte Carlo track simulation to develop a model of AP site induction by irradiation. The yields of AP sites analyzed by the simulation are in good agreement with experimental ones. As a result, we newly determined the branching ratio of induction of the AP the site in the simulation.

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