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Report No.

Effects of ion beam irradiation on the expression of glutathione peroxidase in cultured human retinal vascular endothelial cells exposed to L-dopa

Akeo, Kiyoshi*; Hamada, Nobuyuki*; Funayama, Tomoo; Akeo, Yoko*; Kobayashi, Yasuhiko

We examined the influence of L-dopa on the inactivation of reactive oxygen species by glutathione peroxidase (GPx), which protects in human retinal vascular endothelial (RE) cells from free radical damage. We used ion beams to induce oxidative stress and measured GPx expression by means of real-time reverse-transcriptase-polymerase chain reaction (RT-PCR) analysis. Exposure to L-dopa inhibited GPx expression in human RE cells. GPx expression in RE cells incubated with L-dopa decreased significantly after exposure to 50 MeV He and 220 MeV C, disturbing the defense mechanism against the L-dopa-induced production of reactive oxygen species. In contrast, 350 MeV Ne irradiation significantly increased GPx3 expression. The result indicated that there are the influence of ion beams on L-dopa-induced stress rather than the influence of L-dopa on ion beam-induced stress because GPx expression in L-dopa-treated human RE cells depended on the types of heavy ion used during irradiation.



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