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Report No.
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Development of an effective screening system for growing high quality large volume crystals for combined neutron and X-ray crystallography

Shibazaki, Chie; Adachi, Motoyasu; Kuroki, Ryota

For elucidation of the molecular recognition and catalytic mechanism of biomolecules, not only the detailed molecular structure, but also the hydration structure of the catalytic site and the exact position of hydrogen atoms are important. Collaborative use of both neutron and X-ray crystallography is a powerful methodology to obtain this information. In such experiments, high quality single crystals with large volume enable us to obtain high resolution X-ray diffraction by reducing the effect of X-ray damage, and improve neutron diffraction data by increasing diffraction intensity. Two different approaches have been attempted to obtain large crystals. One is the periodic addition of protein solution to the mother liquor, and the other is to increase the volume of mother liquor. We report the result of increase of the mother liquor volume after focusing on additives and pH to control the nucleation of protein crystals using several proteins.

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