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Report No.

A New "On-Dish" method to analyze individual photosynthetic abilities of young plant seedlings

Kawachi, Naoki; Koyanagi, Atsushi*; Suzui, Nobuo; Ishii, Satomi; Yin, Y.-G.; Iwasaki, Aya*; Ogawa, Kenichi*; Fujimaki, Shu

We have developed a method based on $$^{11}$$CO$$_{11}$$ tracer gas and the positron-emitting tracer imaging system (PETIS) to evaluate individual photosynthetic abilities of young plant seedlings planted collectively on a petri dish with agar medium, for analysis studies on effects of genetic modification or treatment of chemical agents on enhancing plant biomass. As a result of evaluation experiments, $$^{11}$$C-images with little variation among the plants were obtained, and the average rate of carbon fixation by wild-type plants was estimated only with approximately 6% of standard error through a mathematical analysis, which proved the good quantitative capability of this experimental system and analysis method for evaluation of a genetically homogenous line. Then, eight plants of ch1-1 mutant, which lacks chlorophyll b and has lower carbon fixation activity, were also subjected to this method together with eight wild-type plants. It was estimated that ch1-1 mutants has a carbon fixation rate approximately 52.6 $$pm$$ 4.1% lower than that of wild-type in average. In conclusion, we realized a method of quantitative and statistical evaluation of carbon fixation for small plant individuals, by using PETIS.



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