Preliminary neutron diffraction analysis of high-potential iron-sulfur protein

Hirano, Yu; Takeda, Kazuki*; Kurihara, Kazuo; Tamada, Taro; Miki, Kunio*

High-potential iron-sulfur protein (HiPIP) is a soluble electron carrier protein that transfers electrons from cytochrome ${it bc$_{1}$}$ complex to the reaction center complex in photosynthetic purple bacteria. We prepared large crystals and examined conditions for diffraction data collection in order to obtain high-resolution neutron structure. We successfully obtained crystals with the volume over 2 mm $^{3}$ by macro-seeding method. Preliminary diffraction analysis was performed at J-PARC, and the HiPIP crystal diffracted to 1.1 resolution that is the highest resolution in the protein neutron diffraction data.

Language	:	Japanese
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Meeting title	:	平成25年度日本結晶学会年会
Held date	:	2013/10
Location (city)	:	熊本
Location (country)	:	日本
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Registration No. : BB20131915
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