Structural characteristics of alkaline phosphatase from the moderately halophilic bacterium sp.593

Arai, Shigeki; Yonezawa, Yasushi*; Ishibashi, Matsujiro*; Matsumoto, Fumiko*; Adachi, Motoyasu; Tamada, Taro; Tokunaga, Hiroko*; Blaber, M.; Tokunaga, Masao*; Kuroki, Ryota

In order to clarify the structural basis of halophilic characteristics of an alkaline phosphatase derived from the moderate halophile sp.593 (HaAP), the tertiary structure of HaAP was determined to 2.1 resolution by X-ray crystallography. Structural properties of surface negative charge and core hydrophobicity are shown to be intermediate between halophile and non-halophile characteristics, and may explain the unique functional adaptation to a wide-range of salt concentration.

Language	:	English
Journal	:	Acta Crystallographica Section D
Volume	:	70
Number	:	3
Pages	:	p.811 - 820
Publication Year/Month	:	2014/03
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Paper URL	:	https://doi.org/10.1107/S1399004713033609
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Keywords	:	no keyword
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Article of JAEA R&D Review	:	Toward the Development of Protein Absorbents for Rare and Harmful Metals; Structural Characteristics of Halophilic Proteins Clarified by a Quantum Beam[/]
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Registration No. : AA20130838
JAEA Abstracts No. : 42000603
Paper Submission No. : 14229

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