Measurement of radical scavenging activity of irradiated Kampo extracts using ESR spin-trap method

Ota, Yui*; Kawamura, Shoei*; Kikuchi, Masahiro; Kobayashi, Yasuhiko; Ukai, Mitsuko*; Nakamura, Hideo*

The radical scavenging activity (RSA) of 13 kinds of -ray irradiated Kampo extracts were studied by ESR spin-trap method. The RSA against alkoxy radical and hydroxyl radical were measured using new spin trapping reagent CYPMPO. The RSA against these two radicals were evaluated using GSH for alkoxy RSA and L-ascorbic acid for hydroxy RSA as a standard antioxidant reagent. We revealed that a few Kampo extracts showed high RSA against alkoxy radical and hydroxy radical. This RSA of Kampo extracts was changed by -ray irradiation treatment. Using ESR spin-trap method, it is concluded that the effect of radiation treatment on RSA of Kampo extracts were able to detect.