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Molecular analysis of heavy ion induced mutations in budding yeast ${it S. cerevisiae}$

Matsuo, Yoichiro*; Izumi, Yoshinobu*; Hase, Yoshihiro; Sakamoto, Ayako; Nozawa, Shigeki; Narumi, Issey*; Shimizu, Kikuo*

To investigate the nature of mutations induced by accelerated ions in eukaryotic cells, the effects of carbon-ion irradiation were compared with those of $$gamma$$-ray irradiation in the budding yeast ${it Saccharomyces cerevisiae}$. Previous studies suggested that the mutation sites induced by carbon ions were localized near the linker regions of nucleosomes, whereas mutations induced by $$gamma$$ rays were located uniformly throughout the gene. We hypothesized that the locus of mutations might be related to the nucleosome structure. To confirm this hypothesis, we examined the mutation spectrum in the ${it URA3}$ gene with the altered nucleosome structure. It is likely that sites of mutations occurred in the ${it URA3}$ with altered nucleosome structure is inconsistent with those in the wild type. We will further accumulate the data to examine the above hypothesis.



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