Genetic analysis of novel DNA cross-link repair genes common to and

放射線抵抗性細菌と高度好熱菌に共通する新規DNA鎖間架橋修復遺伝子に関する遺伝的解析

小野寺 威文*; 佐藤 勝也; 太田 敏博*; 鳴海 一成*

Onodera, Takefumi*; Sato, Katsuya; Ota, Toshihiro*; Narumi, Issey*

The and orthologs are highly conserved in eubacteria, and the former is also widely found in the genomes of archaea and eukaryotes. However, their functions are still poorly understood. and possess the both orthologs hereafter , , and , respectively. We have reported that the and disruptants show extreme sensitivity to mitomycin C (MMC), which causes interstrand DNA cross-links. In this study, we constructed and gene expression plasmids and the resultant plasmids were introduced into the and disruptant strains. To investigate whether and orthologs share the common function in and , these generated strains were challenged by MMC.