Benomyl-tolerant mutation of entomopathogenic fungi induced by carbon ion beams

Saito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*

In order to identify the mutation sites of -tubulin in the benomyl-tolerant mutants, we determined the nucleotide sequences of -tubulin locus and compared with those of the wild-type strains. In , the -tubulin sequences were identical to that of the wild-type, suggesting that other mechanisms might be responsible for the benomyl tolerance. On the other hand, in , the mutation was identified to be an A:T to C:G transversion at position 924 in the -tubulin gene. This mutation causes an amino acid substitution at position 198 (Glu to Ala) in the -tubulin protein. This site might be a binding target of benomyl or adjacent to the center of benomyl interaction with the -tubulin. These results suggest that mechanisms of benomyl tolerance were different between and .