Simultaneous saccharification and fermentation from ionic liquid-pretreated biomass using ionic liquid-tolerant yeast mutant

イオン液体耐性酵母を用いたイオン液体前処理バイオマスからの同時糖化発酵

仁宮 一章*; 表 小百合*; 佐藤 勝也; 鳴海 一成*; 清水 宣明*

Ninomiya, Kazuaki*; Omote, Sayuri*; Sato, Katsuya; Narumi, Issey*; Shimizu, Nobuaki*

In simultaneous saccharification and fermentation of biomass, pretreatment using ionic liquid has received a lot of attention in recent years. A enormous quantities of wastewater become a problem in washing from ionic liquid-pretreated biomass because ionic liquid is highly poisonous. In this study, simultaneous saccharification and fermentation from ionic liquid-pretreated biomass was carried out using ionic liquid-tolerant yeast that was obtained from the mutant population prepared by irradiation with carbon ion beams (220 MeV C, 100 Gy). By using the ionic liquid-tolerant yeast strain, we could simplify the washing procedure of ionic liquid-pretreated biomass prior to the simultaneous saccharification and fermentation.