Simultaneous saccharification and fermentation from ionic liquid-pretreated biomass using ionic liquid-tolerant yeast mutant

Ninomiya, Kazuaki*; Omote, Sayuri*; Sato, Katsuya; Narumi, Issey*; Shimizu, Nobuaki*

In simultaneous saccharification and fermentation of biomass, pretreatment using ionic liquid has received a lot of attention in recent years. A enormous quantities of wastewater become a problem in washing from ionic liquid-pretreated biomass because ionic liquid is highly poisonous. In this study, simultaneous saccharification and fermentation from ionic liquid-pretreated biomass was carried out using ionic liquid-tolerant yeast that was obtained from the mutant population prepared by irradiation with carbon ion beams (220 MeV C, 100 Gy). By using the ionic liquid-tolerant yeast strain, we could simplify the washing procedure of ionic liquid-pretreated biomass prior to the simultaneous saccharification and fermentation.