-ray sensitivity in exhibiting different flavonoid accumulation patterns

異なるフラボノイド蓄積パターンを示すシロイヌナズナにおける線感受性

北村 智; 鳴海 一成*

Kitamura, Satoshi; Narumi, Issey*

フラボノイドの植物体内効果を詳しく知るために、異なるフラボノイド蓄積パターンを示すシロイヌナズナを用いて、線感受性を評価した。野生型とフラボノイド完全欠損変異体の乾燥種子を照射し、フラボノイド欠損種子で線感受性が増大していることを突き止めた。種子特異的なフラボノイド変異体を同様に線照射したところ、野生型よりも線感受性が高まっていた。種子における活性酸素消去能を評価し、乾燥種子の線感受性が高い系統ほど、活性酸素消去能が低いことが示唆された。

In this study, to understand more deeply the physiological functions of flavonoids in plants, we analyze the responses to -irradiation in Arabidopsis thaliana exhibiting different flavonoid accumulation patterns. When dry seeds were exposed to -rays, obvious differences in -ray sensitivities were detected between WT and a complete flavonoid-less mutant, tt4. When dry seeds of tt12, seed-specific flavonoid mutant, were exposed to -rays, a survival rate was also reduced compared to WT. In addition, to know the relationship between -ray sensitivity and radical scavenging activity of flavonoids, ROS scavenging ability in dry seeds was determined for each genotype. These results suggest that flavonoids would contribute ROS scavenging within irradiated seeds, even in the case where the flavonoid accumulating tissues are apart from the rudimentary plant body, an embryo.