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Discovery of Cs selective binding site on a halophilic beta-lactamase by the anomalous X-ray diffraction analysis

Arai, Shigeki; Adachi, Motoyasu; Kawamoto, Masahide*; Tokunaga, Hiroko*; Ishibashi, Matsujiro*; Tokunaga, Masao*; Kuroki, Ryota

We attempted to discover the Cs$$^{+}$$ and Sr$$^{2+}$$ binding sites on the halophilic $$beta$$-Lactamase (HaBLA) derived from ${it Chromohalobacter}$ sp.560 by the anomalous X-ray diffraction analysis. One Cs$$^{+}$$ ions in the HaBLA crystal were identified by BL7 at SAGA-LS. Three Sr$$^{2+}$$ ions in the HaBLA crystal were identified by BL38B1 at SPring-8 and NW12A at Photon Factory. Discovered Cs$$^{+}$$ binding site can bind Cs$$^{+}$$ selectively from a solution containing Na$$^{+}$$/Cs$$^{+}$$ = 90 mM/10 mM. This Cs$$^{+}$$ binding site is formed by two main-chain O atoms and an aromatic ring of a side chain of Trp. An aromatic ring of Trp interacts with Cs$$^{+}$$ by the cation-$$pi$$ interaction. The observation of metal binding sites with relatively higher Cs$$^{+}$$ selectivity provides important information that is useful for designing artificial Cs$$^{+}$$ binding sites.

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Japanese

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第14回日本蛋白質科学会年会

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2014/06

Location (city)

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横浜

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日本

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no keyword

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Press Release

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Article of JAEA R&D Review

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- Accesses

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