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Intense laser-plasma soft-X-ray sources and its application to biological X-ray microscopy

Kado, Masataka; Kishimoto, Maki; Ejima, Takeo*; Tamotsu, Satoshi*; Yasuda, Keiko*; Aoyama, Masato*; Shinohara, Kunio*

Soft X-ray microscope has a potential to observe live biological cells. But the cells had to be frozen in order to avoid radiation effects onto the biological cells or to be made a short pulse imaging with an intense X-ray source. Otherwise live cell imaging was not realized. Laser-plasma soft X-ray source was extremely bright and had short pulse duration. Hence it makes possible to capture soft X-ray images of biological cells before the cells damaged. Since the spatial resolution of the soft X-ray microscope depends on the photon flux irradiated onto the samples, it is important to increase brightness of the soft X-ray sources to observe inner structures of biological cells. We have increased the brightness of the soft X-ray source and succeeded in observing inner structures of live biological cells.

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