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Analysis of medium-mediated bystander effects induced by carbon-ion between human lung normal and cancer cells

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Kobayashi, Yasuhiko; Kanai, Tatsuaki*

The purpose of this research is to analyze carbon-ion induced bystander effects via medium, and is to understand a characteristic phenomenon between different type cells. In this research, human lung normal fibroblasts WI-38 and human lung cancer cells H1299/wt${it p53}$ were used. Cells were irradiated with carbon-ion broad beams (LET=108 keV/$$mu$$m, Dose=0.13, 0.5 Gy), then survival rates of bystander cells after 6- or 24-hours co-culture with irradiated cells were measured using colony formation assay. When dose was changed, we found that bystander responses are also different between irradiated WI-38 and non-irradiated H1299/wt${it p53}$.

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