Metabolome analysis of leaves of L. irradiated with ion beams

イオンビーム照射したエゾノギシギシの葉のメタボローム解析

宮城 敦子*; 北野 沙也佳*; 大野 豊; 長谷 純宏; 川合 真紀*; 大野 豊

Miyagi, Atsuko*; Kitano, Sayaka*; Ono, Yutaka; Hase, Yoshihiro; Kawai, Maki*; Ono, Yutaka

(Polygonaceae) is a weed, which spreads all over the world. It contains amino acids and ascorbate (Vitamin C) more than other edible species (Miyagi et al., 2010). On the other hand, it highly accumulates soluble oxalate, which causes renal syndrome for vertebrates. Thus we aimed to lower oxalate contents in R. obtusifolius that can be used as a pasture by radiation breeding. In the present study, plant seeds irradiated with several doses of carbon ion beams (C) were used to analyze effects of the radiation to oxalate contents and other metabolites using capillary electrophoresis-mass spectrometry (CE-MS). Results showed that the oxalate contents of leaves were increased by the irradiation of ion beams on the contrary to the case of the irradiation with rays. High oxalate plants tended to contain high levels of organic acids. Amino acids and phosphorylated organic acids were decreased in these plants.