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Development of cesium-accumulating bacteria by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro; Hase, Yoshihiro; Narumi, Issey*; Ono, Yutaka

Bioremediation uses biological organisms to solve an environmental problem. However, microorganisms are sometimes not enough effective to achieve efficient recovery. Therefore, enhancing the functionality of microorganisms is needed to promote the active use of bioremediation. This study began with the purpose of development of Cs-accumulating bacteria by ion beam breeding technology. We investigated which members are better suited for Cs-accumulation in the genus ${it Deinococcus}$ (${it D. radiodurans}$, ${it D. grandis}$, ${it D. proteolyticus}$, ${it D. radiopugnans}$, ${it D. geothermalis}$ and ${it D. murrayi}$) exhibit extraordinary radioresistant. The Cs concentration in the ${it Deinococcus}$ cells exhibited over 4-fold compared with that of the ${it E. coli}$ by atomic absorption spectrometer analysis. This result suggested that ${it D. radiodurans}$ and ${it D. grandis}$ are best two suitable species for the development of high Cs-accumulating bacteria by ion beam breeding technology.

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