Target irradiation of cells and individuals with heavy-ion microbeams of JAEA-Takasaki

Funayama, Tomoo; Yokota, Yuichiro; Sakashita, Tetsuya; Suzuki, Michiyo; Ikeda, Hiroko; Kobayashi, Yasuhiko

In JAEA-Takasaki, there are two microbeam systems for biological study: collimating heavy-ion microbeam system, and focusing heavy-ion microbeam system. Using the collimating microbeam system, we have carried out the cultured-cell studies of heavy-ion hit effect and analysis of bystander effects. Moreover, we have promoted the microbeam studies of small individuals, such as , silkworm, and medaka fish. In these studies, we carried out not only researches aimed to explore the effects of heavy-ion radiation, but also the researches of physiological or developmental biology by applying microbeam as the tool of the radio-microsurgery. The focusing microbeam system was developed for overcoming the limitations of the collimating system. Using the system, we developed the experimental system to irradiate cells rapidly and accurately with the scanned microbeam spot of high-energy heavy-ions.