Arabidopsis MATE-type transporter gene FFT/DTX35 is involved in anthocyanin accumulation in seed coat

シロイヌナズナのMATEトランスポーターFFT/DTX35は種皮のアントシアニン蓄積に関与する

北村 智; 大野 豊; 鳴海 一成*

Kitamura, Satoshi; Ono, Yutaka; Narumi, Issey*

今までほとんど分かっていなかった、シロイヌナズナにおけるアントシアニン蓄積機構を解明するために、banyuls変異体にイオンビームを再照射し、種子の赤色色素蓄積が低下した変異体pab1を得た。分子生物学的解析から、pab1の原因遺伝子は、シロイヌナズナ種皮のアントシアニン蓄積を支配する遺伝子であると考えられた。

Despite intensive investigation on flavonoid biosynthesis, anthocyanin transport and accumulation mechanisms are not fully understood in Arabidopsis. In this study, using an Arabidopsis mutant banyuls (ban), which accumulates anthocyanins instead of colorless proanthocyanidin (PA) precursors in immature seeds, novel mutants were screened based on differences in pigmentation of immature seeds. One of the mutants, pale banyuls1 (pab1), showed a reduced level of anthocyanins in immature seeds compared to ban. Map-based cloning suggested that a MATE-type transporter gene FFT/DTX35 was disrupted in two independent pab1 mutants. Molecular complementation of pab1 with a functional FFT/DTX35 gene provides evidence that the mutation in FFT/DTX35 is responsible for the anthocyanin-less phenotype in immature seeds of pab1.