QENS of protein solutions measured by the TOF near backscattering spectrometer DNA

Nakagawa, Hiroshi   ; Saio, Tomohide*; Oda, Takashi*; Sato, Mamoru*; Inoue, Rintaro*; Sugiyama, Masaaki*; Tominaga, Taiki*; Kawakita, Yukinobu  

Protein is thermally fluctuating in solution, and the dynamics is essential for its biological functions. A protein has hierarchal structure and dynamics in temporal and spatial scale. In this work, QENS of a multi-domain protein, MurD, were measured by the TOF near backscattering spectrometer DNA in order to observe the internal motions. Hef is classified as an intrinsically disordered protein, which lost the rigid folded domain structure, and then have a more flexible structure than a folded protein. We will discuss the data treatment and analytical method of QENS of protein solutions, and characteristic dynamical features of folded rigid and disordered flexible proteins in the presentation.