Soft, skin-interfaced microfluidic systems with integrated immunoassays, fluorometric sensors, and impedance measurement capabilities

Kim, S.*; Lee, B.*; Reeder, J. T.*; Seo, S. H.*; Lee, S.-U.*; Hourlier-Fargette, A.*; Shin, J.*; Sekine, Yurina   ; Jeong, H.*; Oh, Y. S.*; Aranyosi, A. J.*; Lee, S. P.*; Model, J. B.*; Lee, G.*; Seo, M.-H.*; Kwak, S. S.*; Jo, S.*; Park, G.*; Han, S.*; Park, I.*; Jung, H. I.*; Ghaffari, R.*; Koo, J.*; Braun, P. V.*; Rogers, J. A.*

In this study, we present a wireless, battery-free, skin-interfaced microfluidic system that combines lateral flow immunoassay for sweat cortisol assay, fluorometric imaging of glucose and ascorbic acid (vitamin C) assays, and digital tracking of sweat rate using electrodes that measure skin galvanic response. Systematic benchtop testing and on-body field studies on human subjects exercising in a gym environment highlight the key multifunctional features of this platform in tracking the biochemical correlates of physical stress.