Analysis of radiation induced mutation in organ cells derived from human induced pluripotent stem cells

Shimada, Mikio*; Kanzaki, Norie   ; Yanagihara, Hiromi*; Miyake, Tomoko*; Matsumoto, Yoshihisa*

Although mutation frequency depends on organ cell types and differentiation level, it is not fully understood that organ cell types dependent mutation frequency in human cells. In this study, we aimed to establish measurement system of radiation dependent mutation frequency for analyze radiation effect to the human body. For this purpose, we derived four different organ cells such as neural cells, skin keratinocytes, heart muscle cells and blood cells from hiPSCs. Further, using artificial intelligence technology and machine leaning method, we w analyzed differences of mutation frequency during samples.