Polydopamine-Coating Nanoarchitectonics of Cellulose Hydrogels with Promoted Sustainable and Alkali-Resistant Natures for 3D Cell Culture Application

アルカリ耐性と持続性を付与したセルロースハイドロゲルへのポリドーパミンコーティング・ナノアーキテクトニクス; 3次元細胞培養への応用

関根 由莉奈   ; 本田 みちよ*; 杉田 朝子; 南川 卓也 ; 坂中 鈴実*; 深澤 倫子*; 有賀 克彦*

Sekine, Yurina; Honda, Michiyo*; Sugita, Asako; Nankawa, Takuya; Sakanaka, Rimi*; Fukazawa, Tomoko*; Ariga, Katsuhiko*

Sustainable and chemically stable hydrogels are essential for biointerface engineering under alkaline conditions. We developed an alkali-resistant hydrogel from carboxymethyl cellulose nanofibers (CMCF) using freeze-drying and mild heating, which induces partial esterification and densifies the network while maintaining porosity. The resulting hydrogels remain stable at pH 9.7 and allow uniform polydopamine (PDA) coating at pH 8.5. The PDA-coated hydrogels exhibit high water content, robust stability, and excellent biocompatibility, supporting 3D culture of human fibroblasts for over 30 days. This green and versatile platform offers potential for regenerative medicine and environmental applications.