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Journal Articles

Genetic dissection of hormonal responses in the roots of arabidopsis grown under continuous mechanical impedance

Okamoto, Takashi*; Tsurumi, Seiji*; Shibasaki, Kyohei*; Obana, Yoshimi*; Takaji, Hironori*; Ono, Yutaka; Rahman, A.*

Plant Physiology, 146(4), p.1651 - 1662, 2008/04

 Times Cited Count:50 Percentile:77.04(Plant Sciences)

The Arabidopsis seedlings grown horizontally on a dialysis membrane-covered agar plate encountered adequate mechanical impedance as the roots showed characteristic ethylene phenotypes: 2-fold reduction in root growth, increase in root diameter, decrease in cell elongation, and ectopic root hair formation. The root phenotype characterization of various mutants having altered response to ethylene biosynthesis or signaling, the effect of ethylene inhibitors on mechanically impeded roots, and transcription profiling of the ethylene-responsive genes led us to conclude that enhanced ethylene response plays a primary role in changing root morphology and development during mechanical impedance. Further, the differential sensitivity of horizontally and vertically grown roots toward exogenous ethylene suggested that ethylene signaling plays a critical role in enhancing the ethylene response. We subsequently demonstrated that the enhanced ethylene response also affects the auxin response in roots. Taken together, our results provide a new insight into the role of ethylene in changing root morphology during mechanical impedance.

Oral presentation

Continuous gravitropic and mechanical stimulations enhance ethylene response rather than ethylene production in Arabidopsis roots

Okamoto, Takashi*; Rahman, A.*; Takaji, Hironori*; Ono, Yutaka; Tsurumi, Seiji*

no journal, , 

Growth of horizontally grown Arabidopsis thaliana seedlings was almost half (0.57-fold) as compared to that of vertically grown ones. Comparison of the root growth analysis ethylene mutants has showed that ethylene may play an important role in this process. No significant difference was found in ethylene production between horizontal and vertical, suggesting that the reduced elongation of horizontal roots was attributed to the ethylene response rather than the ethylene production. This model is further supported by the root growth behavior of ethylene overproduction mutant and constitutive triple response mutant. In addition, the estimation of ethylene responsive genes by real-time PCR showed marked enhancement of the expression in horizontal roots; 5.2-fold for Ethylene Responsive Factor, ERF and 2.7-fold for basic chitinase, BACH, respectively.

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