検索対象:     
報告書番号:
※ 半角英数字
 年 ~ 
 年
検索結果: 2 件中 1件目~2件目を表示
  • 1

発表形式

Initialising ...

選択項目を絞り込む

掲載資料名

Initialising ...

発表会議名

Initialising ...

筆頭著者名

Initialising ...

キーワード

Initialising ...

使用言語

Initialising ...

発行年

Initialising ...

開催年

Initialising ...

選択した検索結果をダウンロード

論文

Nitrogen K-edge X-ray absorption near edge structure of pyrimidine-containing nucleotides in aqueous solution

島田 紘行*; 南 寛威*; 奥泉 直人*; 佐久間 一郎*; 鵜飼 正敏*; 藤井 健太郎; 横谷 明徳; 福田 義博*; 斎藤 祐児

Journal of Chemical Physics, 142(17), p.175102_1 - 175102_9, 2015/05

AA2015-0073.pdf:1.15MB

 被引用回数:10 パーセンタイル:38.84(Chemistry, Physical)

X-ray absorption near edge structure (XANES) was measured at energies around the N K-edge of the pyrimidine-containing nucleotides cytidine 5'-monophosphate (CMP), 2'-deoxythymidine 5'-monophosphate (dTMP), and uridine 5'-monophosphate (UMP) in aqueous solutions and in dried films under various pH conditions. The features of resonant excitations below the N K-edge in the XANES spectra for CMP, dTMP, and UMP changed depending on the pH of the solutions. The spectral change thus observed is systematically explained by the chemical shift of the core-levels of N atoms in the nucleobase moieties caused by structural changes due to protonation or deprotonation at different proton concentrations. This interpretation is supported by the results of theoretical calculations using density functional theory for the corresponding nucleobases in the neutral and protonated or deprotonated forms.

口頭

pH dependence of soft X-ray absorption spectra of nucleotides in aqueous solution

島田 紘行*; 南 寛威*; 佐久間 一郎*; 奥泉 直人*; 鵜飼 正敏*; 藤井 健太郎; 横谷 明徳; 福田 義博*; 斎藤 祐児

no journal, , 

In order to investigate the role of core-excited states induced by X-ray absorption of DNA under biological environments, X-ray absorption near edge structure (XANES) at N K-edge region were measured for various nucleotides in aqueous solutions. The experiments were performed at BL-23SU in SPring-8 using a liquid microjet apparatus. pH of the sample solution was controlled by addition of NaOH. Total yield of electrons ejected from the sample solution by irradiation was measured as a function of X-ray energies. Under neutral conditions, the XANES spectrum for GMP shows two peaks (A) at lower energy and (B) at higher energy with similar intensities in the nitrogen pre-K edge region. In contrast, under basic condition, the intensity of peak (A) increases, while that of (B) decreases. The pH dependence was also observed for other nucleotides. These results show that specific sites of N atoms in the nucleobase moieties could be (de)protonated depending on the pH conditions.

2 件中 1件目~2件目を表示
  • 1