Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from

田口 千穂; 田浦 太志*; 玉田 太郎; 正山 祥生; 正山 征洋*; 田中 宏幸*; 黒木 良太; 森元 聡*

Acta Crystallographica Section F, 64(3), p.217 - 220, 2008/03

https://doi.org/10.1107/S1744309108003096

Polyketide synthase-1 (PKS-1) is a novel type III polyketide synthase that catalyzes biosynthesis of the hexanoyl triacetic acid lactone in (Mexican strain). PKS-1 was overproduced in , purified and finally crystallized in two different space groups. The crystal obtained in 0.1 M HEPES buffer (pH 7.5) containing 0.2 M calcium acetate and 20% (w/v) polyethylene glycol 3350, diffracted to 1.65 resolution and belonged to space group 1 with unit-cell parameters of a = 54.3 , b = 59.3 , c = 62.6 , = 69, = 81, = 80. Another crystal obtained in 0.1 M HEPES buffer (pH 7.5) containing 0.2 M sodium chloride and 20% (w/v) polyethylene glycol 3350, diffracted to 1.55 resolution and belonged to the space group 222 with unit-cell parameters of a = 54.3 , b = 110 , c = 130 . These data will enable us to determine the crystal structure of PKS-1.