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論文

Clades of huge phages from across Earth's ecosystems

Al-Shayeb, B.*; Sachzdeva, R.*; Chen, L.-X.*; Ward, F.*; Munk, P.*; Devoto, A.*; Castelle, C. J.*; Olm, M. R.*; Bouma-Gregson, K.*; 天野 由記; et al.

Nature, 578(7795), p.425 - 431, 2020/02

 被引用回数:20 パーセンタイル:0.48(Multidisciplinary Sciences)

Phage typically have small genomes and depend on their bacterial hosts for replication. We generated metagenomic datasets from many diverse ecosystems and reconstructed hundreds of huge phage genomes, between 200 kbp and 716 kbp in length. Thirty four genomes were manually curated to completion, including the largest phage genomes yet reported. Expanded genetic repertoires include diverse and new CRISPR-Cas systems, tRNAs, tRNA synthetases, tRNA modification enzymes, initiation and elongation factors and ribosomal proteins. Phage CRISPR have the capacity to silence host transcription factors and translational genes, potentially as part of a larger interaction network that intercepts translation to redirect biosynthesis to phage-encoded functions. Some phage repurpose bacterial systems for phage-defense to eliminate competing phage. We phylogenetically define seven major clades of huge phage from human and other animal microbiomes, oceans, lakes, sediments, soils and the built environment. We conclude that large gene inventories reflect a conserved biological strategy, observed across a broad bacterial host range and resulting in the distribution of huge phage across Earth's ecosystems.

口頭

Vast metabolic and phylogenetic diversity shared across deep subsurface environments

天野 由記; Diamond, S.*; Lavy, A.*; Anantharaman, K.*; 宮川 和也; 岩月 輝希; 別部 光里*; 鈴木 庸平*; Thomas, B. C.*; Banfield, J. F.*

no journal, , 

We investigated the microbiology two Japanese subsurface research sites and compared the major groups of organisms lacking cultivated representatives found from other subsurface sites, including a Colorado aquifer and deep aquifers underlying the Crystal Geyser. We analyzed metagenomic data 19 samples from the Horonobe site and 7 from the Mizunami site. DNA sequences from each sample were assembled independently and scaffolds encoding the ribosomal protein S3 sequence were identified. The major characteristic of the microbiology of the Mizumani site that distinguished it from the Horonobe site is local very high abundances of Nitrospirae, Parcubacteria, Ignavibacteria, ANME-2D and Micrarchaeota. In contrast, the Horonobe site has locations that are highly enriched in Altarchiales, Syntrophobacteriales, Atribacteria, ANME-2D and Methanogens. Beyond reshaping the Tree of Life, the societal importance of these discoveries remains little known. However, given the huge inventory of new groups of proteins and pathways in the genomes of these organisms, it is reasonable to anticipate major discoveries will hold relevance, for example, in terms of pharmaceutical discovery. Given the importance of the subsurface as a potential host environment for storage of nuclear waste, finding some commonality would indicate the general relevance of information from one site for prediction of the characteristics of other sites.

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