検索対象:     
報告書番号:
※ 半角英数字
 年 ~ 
 年
検索結果: 3 件中 1件目~3件目を表示
  • 1

発表形式

Initialising ...

選択項目を絞り込む

掲載資料名

Initialising ...

発表会議名

Initialising ...

筆頭著者名

Initialising ...

キーワード

Initialising ...

使用言語

Initialising ...

発行年

Initialising ...

開催年

Initialising ...

選択した検索結果をダウンロード

論文

Quantitative analysis of the initial transport of fixed nitrogen in nodulated soybean plants using $$^{15}$$N as a tracer

Hung, N. V. P.*; 渡部 詩織*; 石川 伸二*; 大竹 憲邦*; 末吉 邦*; 佐藤 孝*; 石井 里美; 藤巻 秀; 大山 卓爾*

Soil Science and Plant Nutrition, 59(6), p.888 - 895, 2013/12

 被引用回数:4 パーセンタイル:14.11(Plant Sciences)

The quantitative analysis of the initial transport of fixed $$^{15}$$N in intact nodulated soybean plants was investigated at the vegetative stage and pod-filling stage by the $$^{15}$$N pulse-chase experiment. The nodulated roots were exposed to N$$_{2}$$ gas labeled with a stable isotope $$^{15}$$N for 1 hour. Plant roots and shoots were separated into three sections with the same length of the main stem or primary root. Approximately 80% and 92% of fixed N was distributed in the basal part of the nodulated roots at vegetative and pod-filling stage the end of 1 hour of $$^{15}$$N$$_{2}$$ exposure, respectively. In addition, about 90% of fixed $$^{15}$$N was retained in the nodules and 10% was exported to root and shoot after 1 hour of $$^{15}$$N$$_{2}$$ exposure at pod-filling stage. The percentage distribution of $$^{15}$$N in the nodules at pod-filling stage decreased from 90% to 7% during the 7 hours of the chase-period, and increased in the roots (14%), stems (54%) leaves (12%), pods (10%), and seeds (4%). The $$^{15}$$N distribution was negligible in the distal root segment, suggesting that nitrogen fixation activity was negligible and recycling fixed N from the shoot to the roots was very low in the initially short time of the experiment.

口頭

$$^{15}$$Nによるダイズ根粒における固定窒素の転流の定量的解析

Hung, N. V. P.; 渡部 詩織*; 石川 伸二*; 大竹 憲邦*; 末吉 邦*; 石井 里美; 藤巻 秀; 大山 卓爾*; 八島 裕幸*

no journal, , 

ダイズ等のマメ科植物にとって、根粒菌による窒素固定は重要な窒素の獲得機構である。根粒菌による窒素固定、及び固定された窒素化合物の植物内の転流を、窒素15をトレーサとして二つの異なる生育ステージにおいて調べた。窒素15標識窒素ガスを播種後36日、91日のダイズの地下部に投与し、トレーサ投与後1-8時間において2時間ごとにサンプリングし、根粒,葉,茎,根などにおける部位別の固定量を質量分析装置によって調べた。その結果、どちらの生育ステージでも根粒からの固定窒素の流出割合は、最初の1時間では1割程度とかなり少ないが、その後、各器官への移行が活発になり、特に地上部への移行割合が8時間後には5割に達した。根では根の上部に着生した根粒の活性が最も高く、固定窒素の根下部への移動はほとんどないことが確認された。これらの結果は播種後日数によらず、同じ傾向であった。

口頭

Analysis of the initial transport of fixed nitrogen and the effect of O$$_{2}$$ partial pressure on nitrogen fixation in soybean using nitrogen isotopes

Hung, N. V. P.; 渡部 詩織*; 石川 伸二*; 大竹 憲邦*; 末吉 邦*; 佐藤 孝*; 石井 里美; 鈴井 伸郎; 河地 有木; 尹 永根; et al.

no journal, , 

The quantitative analysis of initial transport of fixed nitrogen in intact nodulated soybean plants was conducted by the $$^{15}$$N pulse-chase experiment. Approximately 90% of fixed $$^{15}$$N was retained in the nodules and the $$^{15}$$N distribution in the basal nodules (78%) was higher than that of in the middle (12%) and distal nodules (0.1%) after 1 hour of stable isotope $$^{15}$$N exposure. The distribution of fixed $$^{15}$$N in the nodules decreased from 90% to 7% and increased in the other part during the initial 7 hours of the chase-period. The impacts of oxygen in rhizosphere on the symbiotic nitrogen fixation in real-time was evaluated under various O$$_{2}$$ partial pressure. Soybean nodules were treated with mixed gas containing N$$_{2}$$ labeled with a radioisotope $$^{13}$$N and various concentrations of O$$_{2}$$, and the nitrogen fixation activity in the nodules was visualized by the positron-emitting tracer imaging system (PETIS). The results showed that under normal condition the nitrogen fixation ability of soybean plant was higher compared to that of under the other conditions (0% O$$_{2}$$ and 10% O$$_{2}$$). There was no significant difference in the nitrogen fixation activity between 0% O$$_{2}$$ and 10% O$$_{2}$$.

3 件中 1件目~3件目を表示
  • 1