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土橋 邦生*; 清水 泰生*; 松崎 晋一*; 長嶺 竹明*; 佐藤 隆博; 大久保 猛; 横山 彰人; 石井 保行; 神谷 富裕; 荒川 和夫*; et al.
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 87, 2012/01
In-air microparticle-induced X-ray emission (in-air micro-PIXE) analysis is based on irradiation of specimens with a proton ion microbeam, and has been modified for biological application. Two-dimensional analysis and quantitative analysis using the system confirmed that asbestos induced apoptosis by upregulating Fas expression and also revealed the accumulation of CD163-expressing macrophages in the lungs of patients with asbestosis. By quantitative comparison of the area of Fas or CD163 expression and the Fas- or CD163-negative area in asbestos lung tissue, the harmful levels which caused the expression of Fas or CD163 could be estimated on Silica, Ferrous iron, and Magnesium deposition. These results indicate that the system could be very useful for investigating the pathogenesis of inhaled asbestos-induced immune reactions and for determining harmful levels of asbestos.
松崎 晋一*; 清水 泰生*; 土橋 邦生*; 長嶺 竹明*; 佐藤 隆博; 大久保 猛; 横山 彰人; 石井 保行; 神谷 富裕; 荒川 和夫*; et al.
International Journal of Immunopathology and Pharmacology, 23(1), p.1 - 11, 2010/01
In-air micro-particle induced X-ray emission (in-air micro-PIXE) analysis is based on irradiation of specimens with a proton ion microbeam, and has been utilized for biological application. Two-dimensional analysis and quantitative analysis using the system confirmed that asbestos induced apoptosis byupregulating Fas expression and also revealed the accumulation of CD163-expressing macrophages in the lungs of patients with asbestosis. By quantitative comparison of the area of Fas or CD163 expression and the Fas- or CD163-negative area in asbestos lung tissue, the harmful levels which caused the expression of Fas or CD163 could be estimated on Si, Fe, and Mg (the components of asbestos) deposition. These results indicate that the system could be useful for investigating the pathogenesis of inhaled particle-induced immune reactions and for determining harmful levels of exogenous agents.
土橋 邦生*; 清水 泰生*; 松崎 晋一*; 石塚 全*; 森 昌朋*; 佐藤 隆博; 江夏 昌志; 横山 彰人; 大久保 猛; 石井 保行; et al.
no journal, ,
特発性間質性肺炎の発症の原因を解明するため、患者から気管支鏡により採取した微量組織をmicro-PIXEを用いて測定することにより、粉塵吸入の関与を検討した。自動車駐車場の管理人では、Siに一致して、Fe, Mg, Al, Ti, Co, Cr, V, Caなど多種類の金属が検出され、これらはいずれもディーゼル排気中や高速道路沿いの住民などに多く検出される金属であった。本患者では、職場での粉塵吸入と考えられ、これらの金属が線維化の発症に関与している可能性が示された。このように、micro-PIXEを使用すれば、気管支鏡のような微量検体から、患者の粉塵吸入の有無が推定でき、今まで原因不明とされていた患者の中に、職業性粉塵吸入が原因の患者を判別できる可能性が開かれた。