Enzymatic fabrication of protein-decorated gold nanoparticles by the aid of artificial peptides with gold-binding affinity

二井手 哲平*; 下条 晃司郎; 若林 里衣*; 後藤 雅宏*; 神谷 典穂*

Langmuir, 29(50), p.15596 - 15605, 2013/12

https://doi.org/10.1021/la401327h

We report a new approach for the biofabrication of protein-immobilized gold nanoparticles (AuNPs), using oxidoreductase with gold-binding peptide-tagged recombinant proteins. First, we selected the A3 peptide as a gold binding moiety. The A3 peptide was introduced to the C-terminus of fusion proteins of immunoglobulin G (IgG)-binding domains of protein G and protein A. In the presence of the recombinant protein, the GLD-catalyzed cofactor reduction resulted in the efficient in situ fabrication of AuNPs immobilized with the fusion protein. Moreover, the protein-immobilized AuNPs were shown to have IgG binding activity. This enzyme-assisted one-pot methodology for protein-AuNPs conjugation offers one potent route for the facile fabrication of biomolecule-decorated metal NPs.