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堀江 暁*; 富田 武郎*; 斉木 朝子*; 河野 秀俊; 高 ひかり*; 峯木 礼子*; 藤村 務*; 西山 千春*; 葛山 智久*; 西山 真*
Nature Chemical Biology, 5(9), p.673 - 679, 2009/09
被引用回数:41 パーセンタイル:67.64(Biochemistry & Molecular Biology)Although the latter part of the lysine biosynthesis, conversion of -aminoadipate (AAA) to lysine, of is similar to that of the arginine biosynthesis, enzymes homologous to ArgA and ArgJ are absent in the Thermus pathway. Since ArgA and ArgJ are known to modify the amino group of glutamate to avoid intramolecular cyclization of intermediates, their absence suggests an alternative N-modification system in the pathway. We reconstituted the conversion of AAA to lysine, and revealed that the amino group of AAA is modified with the -carboxyl group of C-terminal Glu54 of a small protein, LysW, and the side-chain of AAA is converted to the lysyl side-chain in LysW-attached forms. Lysine is subsequently liberated from LysW/lysine-fusion. Recognition of the acidic globular domain of LysW by biosynthetic enzymes indicates that LysW acts as a carrier protein or protein scaffold of the biosynthetic enzymes in this system. This study reveals the previously unknown function of a small protein in the primary metabolism.
神原 豊三; 宇野 英郎; 荘田 勝彦; 平田 穣; 庄司 務; 小早川 透; 高柳 弘; 藤村 勤; 森田 守人; 市原 正弘; et al.
JAERI 1045, 11 Pages, 1963/03
この報告書はJRR-2の第1次出力上昇試験後、設計出力10MWの出力上昇までの1つのステップとしての3MW,第2次出力上昇試験について記したものである。試験は昭和36年11月15日から開始され、11月29日に3MWに到達し、3MWでの連続運転を行って12月16日終了した。