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CO
and a positron-emitting tracer imaging system諏訪 竜一*; 石井 里美; 鈴井 伸郎; 石岡 典子; 藤巻 秀; 松橋 信平; 藤田 耕之輔*; 河地 有木
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 109, 2008/11
Recently, Kawachi et al. (2006) established a method for the kinetic analysis of CO for studying photoassimilate transport in a leaf, using PETIS. The method quantitatively analyzes the photoassimilate transport under various light conditions. However, it is important to analyze photoassimilate transport under both dark and light conditions because leaves transport carbon under both these conditions (Mullen et al., 1988). In the present study, we developed a new method to analyze photoassimilate translocation, using PETIS, and CO. This novel method clarified the mechanism underlying photoassimilate translocation from a leaf under the dark condition by noninvasive, repeated imaging experiments, using the same specimen.
C tracer and coupling between source and sink organs諏訪 竜一*; 藤巻 秀; 鈴井 伸郎; 河地 有木; 石井 里美; 阪本 浩一*; Nguyen, N. T.*; 実岡 寛文*; Mohapatra, P. K.*; Moghaieb, R. E.*; et al.
Plant Science, 175(3), p.210 - 216, 2008/09
被引用回数：18 パーセンタイル：45.41(Biochemistry & Molecular Biology)Salinity stress affects photosynthate partitioning between sources and sinks of plants, but how it affects on these systems is less understood. Because sources and sinks are closely knitted, any adverse effect under sub-optimal condition on one part is often misinterpreted for the other. Knowledge on regulation of carbon partitioning is indispensable for stress resistance and good plant growth. In the present study, alteration of carbon partitioning in tomato plants (lycopersicon esculentum L. cv. Momotarou) under saline (NaCl) environment was studied by feeding radioactive C and stable 
C isotopes. Pulse chases were conducted for measuring spatial and temporal distributions of C. C was measured by standard conventional technique, but C distribution was monitored using by PETIS. Salt stress resulted in reduced carbon translocation towards roots. Majority of the photosynthate accumulated in the leaf. We have also observed that the reduction in translocation of carbon occurred well before salt stress symptoms of reduced photosynthesis and plant growth in salt exposed plants. The effect on sink activity also showed by decrease in stem diameter and reduced photosynthetic activity. In addition, PETIS analysis of C translocation indicates that carbon translocation to roots was inhibited under salt conditions without direct effect of leaf Na accumulation and osmotic stress These results suggest that NaCl has direct effects on plants inhibiting carbon partitioning within few hours of salt solution exposure without inhibition of source activity.
諏訪 竜一*; 石井 里美; 河地 有木; 藤巻 秀; 鈴井 伸郎; 須藤 広行*; 松橋 信平; 藤田 耕之輔*
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 123, 2007/02
Carbon is an indispensable nutrition source for crop. Until now, it has been impossible to measure carbon fixation and translocation simultaneously in real time repeatedly in intact plant. Using the technologies of PETIS, we are able to visualize the real time fixation and translocation of carbon in tomato plant. We used CO to monitor localization of fixed C in leaf.
諏訪 竜一*; 石井 里美; 鈴井 伸郎; 石岡 典子; 藤巻 秀; 松橋 信平; 河地 有木; 藤田 耕之輔*
no journal, ,
近年、PETIS及びCOを用いた葉における光合成産物の動態解析が行われている。これらの方法を用いることにより、植物体葉におけるさまざまな光条件下での光合成産物の動態のリアルタイム解析が可能となった。葉からの光合成産物の転流は、昼夜いずれでも行われているため、それぞれの条件における転流の様式について解析を進めていく必要がある。そこで本研究では、PETIS及びCOを用い、繰り返し実験による葉における炭素動態のモニタリングから、明条件と暗条件における転流様式の解析を行った。
