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Attie, O.*; Jayaprakash, A.*; Shah, H.*; Paulsen, I. T.*; 守野 正人*; 高橋 優嘉*; 鳴海 一成*; Sachidanandam, R.*; 佐藤 勝也; 伊藤 政博*; et al.
Genome Announcements (Internet), 2(6), p.e01175-14_1 - e01175-14_2, 2014/11
The alkaliphilic bacterium AV1934, isolated from human feces, recent has indicated novel potassium ion coupling to motility in this extremophile. We report draft sequences that will facilitate an examination of whether that coupling is part of a larger cycle of potassium ion-coupled transporters. This draft genome has 4,095 predicted genes and 4,063 predicted proteins. The whole-genome shotgun project has been deposited in Genbank under the accession no. ALPT02000000. In addition to the genes encoding the MotPS channel that uses either potassium or sodium coupling, two loci encoding multisubunit Mrp-type antiporters, were found. The AV1934 genome reveals no tripartite ATP-independent (TRAP-T family) uptake systems, a major sodium-coupled complement in the other two alkaliphiles. The AV1934 genomic data will enable us to test the hypothesis of a major role of potassium in ion coupling in this extremophile.
藤浪 俊*; 武田 喜代子*; 小野寺 威文*; 佐藤 勝也; 佐野 元彦*; 高橋 優嘉*; 鳴海 一成*; 伊藤 政博*
Genome Announcements (Internet), 2(5), p.e00866-14_1 - e00866-14_2, 2014/09
Calcium-dependent sp. strain TCA20 was isolated from a water sample of a hot spring containing a high concentration of calcium ions. Here, we report the draft genome sequence of this bacterium, which may be the basis for the research of calcium ion homeostasis. The draft genome sequence of sp. strain TCA20 totals 5,631,463 bp in length and is composed of 33 large contigs ( 500 bp) that was deposited at DDBJ/EMBL/Genbank under the accession no. BBIW00000000. The annotation of the draft genome sequence shows that sp. strain TCA20 has a calcium-specific calcium/proton antiporter, ChaA, and a P-type calcium-transporting ATPase, YloB, was identified as calcium transporters. These transporters may also be important for the growth of sp. strain TCA20 under the high concentration of calcium ions.
今澤 陸*; 藤浪 俊*; 高橋 優嘉*; 佐藤 勝也; 鳴海 一成*; 伊藤 政博*
no journal, ,
細胞の生育に大きく関与するCaは、細胞内でその濃度が厳密に調節されている。しかし、原核生物の細胞内Ca濃度の調節機構については、未だ不明な点が多い。本研究では、カルシウム含有量が高い弱アルカリ性温泉地から分離されたCaを生育に要求する sp. TCA20株を用いて、細胞内外のCaサイクルの詳細を理解するために、ゲノム解析を行った。その結果、Ca/Hアンチポーターをコードする遺伝子およびP型Ca-ATPaseをコードする遺伝子のホモログを持つことから、これらの遺伝子群がCaサイクルや生育に重要である可能性が示唆された。