Ion beam irradiation has different influences on superoxide dismutase activity in cultured human retinal vascular endothelial cells between C and He

Akeo, Kiyoshi*; Funayama, Tomoo; Kobayashi, Yasuhiko; Akeo, Yoko*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 80, 2015/03

https://doi.org/10.11484/jaea-review-2014-050

It is known that superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. Ionizing radiation is known to induce oxidative stress through generation of reactive oxygen species resulting in imbalance of the pro-oxidant and antioxidant in the cells, which is suggested to culminate in cell death. Therefore, we measured the activity of SOD in human RE cells exposed to the He- and C-ion beam. The cells collected at 0, 4, 8, and 24 hr after irradiation were extracted by adding the SOD assay buffer to the pellets, and lysed by several cycles of freezing and thawing. The activity of SOD was measured using a modification of the luminol assay. SOD activity decreased according to duration time after irradiation of He-ion, however, in case of C-ion, it increased at 24 hr after irradiation. The result suggested that that there were the differences of the effects by irradiation on SOD activity between He- and C-ion.

The Effects of ultraviolet irradiation and hypoxia on expression of glutathione peroxidase and glyceraldehyde 3-phosphate dehydrogenase in the cultured retinal pigment epithelium

Akeo, Kiyoshi*; Funayama, Tomoo; Hamada, Nobuyuki*; Akeo, Yoko*; Hiramitsu, Tadahisa*; Kobayashi, Yasuhiko

Soshiki Baiyo Kenkyu, 26(3), p.149 - 157, 2007/09

Effects of irradiation on BCL2 and TPR53BP2 expression in the porcine ciliary body

Akeo, Kiyoshi*; Funayama, Tomoo; Ogawa, Akira*; Hamada, Nobuyuki*; Akeo, Yoko*; Kobayashi, Yasuhiko

Experimental Animals, 55(4), p.375 - 381, 2006/07

no abstracts in English

Induction of glutathione peroxidase of retinal pigment epithelial cells and retinal vascular endothelial cells exposed to energetic heavy-ion beams

Akeo, Kiyoshi*; Akeo, Yoko*; Tsubota, Kazuo*; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Effect of ion beam irradiation on expression of glutathione peroxidase in cultured cell of human retinal vascular endothelium

Akeo, Kiyoshi*; Hamada, Nobuyuki*; Funayama, Tomoo; Kobayashi, Yasuhiko; Kawada, Kumiko*; Akeo, Yoko*

no journal, , 

no abstracts in English

Ne, C and He ion beam irradiations have different effects on the expression of glutathione peroxidase in cultured human retinal vascular endothelial cells exposed to L-dopa

Akeo, Kiyoshi*; Hamada, Nobuyuki*; Funayama, Tomoo; Akeo, Yoko*; Kobayashi, Yasuhiko

no journal, , 

We investigate how L-dopa influences glutathione peroxidase (GPX) in preventing damage to membrane phopholipids of retinal endothelial cells (RE). Human RE incubated in vitro with 250 M L-dopa were exposed to heavy ion beams of 350 MeV Ne, 220 MeV C, and 50 MeV He. We collected RE after 0, 4, 8, 24 hrs of irradiation, extracted total cellular RNA, and synthesized cDNA, then the expression of GPX and 18S RNA were measured by realtime RT-PCR using specifically designed primers. The exposure to L-dopa inhibited the expression of GPX in RE. The expression of GPX in RE incubated with L-dopa decreased significantly after exposure to He and C. In contrast, Ne- ion beam irradiation significantly increased the expression of GPX in RE incubated with L-dopa. The result indicated that the difference of LET of ion species resulted to the different expression GPX in RE incubated with L-dopa.