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Saito, Yuki*; Ishiwata, Tobimaru*; Horiuchi, Misato*; Nishiki, Yuto*; Kikuchi, Ryosuke*; Otake, Tsubasa*; Kawakita, Ryohei; Takayama, Yusuke; Mitsui, Seiichiro; Sato, Tsutomu*
Shigen, Sozai Koenshu (Internet), 11(1), 7 Pages, 2024/03
no abstracts in English
Iwata, Keiji*; Imafuku, Muneyuki*; Suzuki, Kanki*; Shobu, Takahisa; Orihara, Hideto*; Sakai, Yusuke*; Akita, Koichi; Oya, Shinichi*; Ishiyama, Kazushi*
Journal of Applied Physics, 117(17), p.17A910_1 - 17A910_4, 2015/03
Times Cited Count：7 Percentile：29.15(Physics, Applied)Internal stress distribution for generating closure domains occurring in laser-irradiated Fe-3%Si(110) steels was investigated using high-energy X-ray analysis and domain theory based on the variational principle. The measured triaxial stresses inside the specimen were compressive and the stress in the rolling direction became more dominant than stresses in the other directions. The calculations based on the variational principle of magnetic energy for closure domains showed that the measured triaxial stresses made the closure domains more stable than the basic domain without closure domains. The experimental and calculation results reveal that the laser-introduced internal stresses result in the occurrence of the closure domains.
Kawasaki, Michio*; Kikyo, Shogo*; Nozawa, Shigeki; Akita, Yusuke*; Hase, Yoshihiro; Narumi, Issey*
Nihon Sakumotsu Gakkai Tohoku Shibu Kaiho, (57), p.61 - 62, 2014/12
no abstracts in English
Hase, Yoshihiro; Akita, Yusuke; Kitamura, Satoshi; Narumi, Issei; Tanaka, Atsushi
Plant Biotechnology, 29(3), p.193 - 200, 2012/06
Times Cited Count：23 Percentile：25.35(Biotechnology & Applied Microbiology)Ion beams have been used as a mutagen to improve the efficiency of plant mutation breeding. Mutation breeding is sometimes perceived as a random process. In this review, we describe our recent progress in developing a more efficient mutagenesis technique using ion beam irradiation combined with sucrose pretreatment or subsequent re-irradiation. To shorten the time required for breeding new cultivars of cyclamen, we identified anthocyanin biosynthesis genes and examined the effectiveness of PCR screening of irradiated deletion-mutant candidates at early growth stages. We believe this research is a step toward more efficient and controlled mutation breeding using ion beams.
-transferase gene in cyclamenKitamura, Satoshi; Akita, Yusuke; Ishizaka, Hiroshi*; Narumi, Issei; Tanaka, Atsushi
Journal of Plant Physiology, 169(6), p.636 - 642, 2012/04
Times Cited Count：85 Percentile：91.26(Plant Sciences)In order to identify the anthocyanin-related GST in cyclamen, four candidates of GSTs (CkmGST1 to CkmGST4) were isolated. Phylogenetic analysis indicated that CkmGST3 was closely related to PhAN9, an anthocyanin-related GST of petunia. Expression analysis at different developmental stages of petals revealed that CkmGST3 was strongly expressed in paler pigmented petals than in fully pigmented petals, in contrast to the constitutive expression of the other three candidates during petal development. This expression pattern of CkmGST3 was correlated with those of other anthocyaninbiosynthetic genes such as CkmF3'5'H and CkmDFR2. Molecular complementation of Arabidopsis 
, a knockout mutant of an anthocyanin-related GST gene, demonstrated that CkmGST3 could complement the anthocyanin-less phenotype of . Transgenic plants that expressed the other three CkmGSTs did not show anthocyanin accumulation. These results indicate CkmGST3 functions in anthocyanin accumulation in cyclamen.
-methyltransferase involved in flower colorationAkita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; et al.
Planta, 234(6), p.1127 - 1136, 2011/12
Times Cited Count：44 Percentile：75.48(Plant Sciences)
spp., generated by ion-beam irraidationKondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 65, 2011/01
no abstracts in English
Tomikawa, Masaru*; Maeda, Tomoo*; Honda, Kazushige*; Saga, Koichi*; Ishikawa, Ryuji*; Akita, Yusuke; Yoshihara, Ryohei; Nozawa, Shigeki; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 73, 2011/01
Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi
Journal of Horticultural Science & Biotechnology, 85(5), p.437 - 443, 2010/09
The flower colour of gra6 (wild-type) is pink-purple in the flower. On the other hand, flowers of 
gra50 (white-flowered variant) exhibit a white colour in its flower. In this study, the relationship between floral pigmentation and expression of several anthocyanin biosynthesis genes was investigated in gra6 and gra50. The pigments in the flower consist mainly of malvidin 3,5-diglucoside in gra6. White-flowered gra50 possessed lower amounts of anthocyanins, but higher amounts of flavonols compared to gra6. Gene expression analysis demonstrated that expression of the dihydroflavonol 4-reductase gene 2 (
) was lower in gra50 compared with gra6, whereas the expression of other key genes did not differ greatly. These results suggest that the white-flowered variant of gra50 may result from a defect in expression of the gene.
spp., generated by ion-beam irradiationKondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
Plant Biotechnology, 26(5), p.565 - 569, 2009/01
Times Cited Count：37 Percentile：68.06(Biotechnology & Applied Microbiology)Fragrant cyclamen cultivar (
), that bloomed purple flower containing malvidin 3,5-diglucoside as the major anthocyanin, was irradiated with a 320-MeV carbon-ion beam at 0-16 Gy to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants irradiated at 2 Gy were flower-color mutants that retained desirable flower shape, flower size, and leaf color. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. Because the major anthocyanins in flowers of spp. were previously restricted to malvidin, peonidin, and cyanidin types, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.
)Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
) by ion beam irradiationKameari, Naoko*; Okubo, Naomi*; Kondo, Emiko*; Nakayama, Masayoshi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
) by ion beam re-irradiationKondo, Emiko*; Kameari, Naoko*; Kitamura, Satoshi; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
) by ion beam irradiationKondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Kitamura, Satoshi; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
Kawasaki, Michio*; Takisawa, Misato*; Kanehira, Saki*; Sakio, Mika*; Honda, Kazushige*; Islam, M. N.*; Suto, Hiroki*; Nozawa, Shigeki; Akita, Yusuke*; Narumi, Issei
no journal, ,
no abstracts in English
Kawasaki, Michio*; Hatta, Yuki*; Senda, Mineo*; Suto, Hiroki*; Nozawa, Shigeki; Akita, Yusuke*; Hase, Yoshihiro; Narumi, Issey*
no journal, ,
no abstracts in English
Kato, Takuno*; Akita, Yusuke*; Ono, Yutaka; Hase, Yoshihiro; Biswas, K. K.*
no journal, ,
no abstracts in English
Yamada, Yusuke*; Kitani, Yuji*; Ikushima, Kazuki*; Shibahara, Masakazu*; Nishikawa, Satoru*; Furukawa, Takashi*; Akita, Koichi; Suzuki, Hiroshi; Morooka, Satoshi
no journal, ,
no abstracts in English
Ishiwata, Tobimaru*; Kikuchi, Ryosuke*; Otake, Tsubasa*; Sato, Tsutomu*; Kawakita, Ryohei; Takayama, Yusuke; Mitsui, Seiichiro
no journal, ,
It is known that the cementation of bentontie may cause the reduction of swelling pressure. However, fundamental mechanism of this reducing swelling pressure has not been well understood. To understand the mechanism, it is essential to identify the cementitious materials and observe the changes in microstructure associated with cementation. In this study, the microstructural observation and swelling tests were conducted on several bentonite ores collected from Tsukinuno Mine in Yamagata Prefecture, Japan, as natural analog of cementation, and the relationship between petrographic texture and swelling pressure was discussed. Thin sections of bentonite ores were prepared by dry polishing technique to observe flat surfaces in micro scales. Scanning electron microscopy (SEM) demonstrated the widespread distribution of authigenic, a few micron silica in the matrix of montmorillonite. Transmission electron microscopy (TEM) revealed that the micro silica minerals (commonly quartz and less commonly amorphous silica) adhered to the edge of montmorillonite grains. These observations suggest that the adherence of these cementitious materials to montmorillonite prevents water access to the interlayer of montmorillonite and swelling, resulting in a decrease in swelling pressure. While these microstructures were observed commonly in all samples collected from several bentonite beds, the relative abundance of montmorillonite and micro silica minerals varied among different beds. It is possible that the swelling pressure in the cemented bentonite is affected by the relative abundance of montmorillonite and cementitious materials.
Tomikawa, Masaru*; Maeda, Tomoo*; Honda, Kazushige*; Saga, Koichi*; Ishikawa, Ryuji*; Akita, Yusuke; Nozawa, Shigeki; Narumi, Issei
no journal, ,
no abstracts in English
