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Journal Articles

Internal stress distribution for generating closure domains in laser-irradiated Fe-3%Si(110) steels

Iwata, Keiji*; Imafuku, Muneyuki*; Suzuki, Kanki*; Shobu, Takahisa; Orihara, Hideto*; Sakai, Yusuke*; Akita, Koichi; Oya, Shinichi*; Ishiyama, Kazushi*

Journal of Applied Physics, 117(17), p.17A910_1 - 17A910_4, 2015/03

 Times Cited Count:5 Percentile:27.89(Physics, Applied)

Internal stress distribution for generating closure domains occurring in laser-irradiated Fe-3%Si(110) steels was investigated using high-energy X-ray analysis and domain theory based on the variational principle. The measured triaxial stresses inside the specimen were compressive and the stress in the rolling direction became more dominant than stresses in the other directions. The calculations based on the variational principle of magnetic energy for closure domains showed that the measured triaxial stresses made the closure domains more stable than the basic domain without closure domains. The experimental and calculation results reveal that the laser-introduced internal stresses result in the occurrence of the closure domains.

Journal Articles

Effects of carbon-ion irradiation on the morphology and growth in the local green soybean "Kemame" in Aomori Prefecture

Kawasaki, Michio*; Kikyo, Shogo*; Nozawa, Shigeki; Akita, Yusuke*; Hase, Yoshihiro; Narumi, Issey*

Nihon Sakumotsu Gakkai Tohoku Shibu Kaiho, (57), p.61 - 62, 2014/12

no abstracts in English

Journal Articles

Development of an efficient mutagenesis technique using ion beams; Toward more controlled mutation breeding

Hase, Yoshihiro; Akita, Yusuke; Kitamura, Satoshi; Narumi, Issei; Tanaka, Atsushi

Plant Biotechnology, 29(3), p.193 - 200, 2012/06

 Times Cited Count:20 Percentile:28.88(Biotechnology & Applied Microbiology)

Ion beams have been used as a mutagen to improve the efficiency of plant mutation breeding. Mutation breeding is sometimes perceived as a random process. In this review, we describe our recent progress in developing a more efficient mutagenesis technique using ion beam irradiation combined with sucrose pretreatment or subsequent re-irradiation. To shorten the time required for breeding new cultivars of cyclamen, we identified anthocyanin biosynthesis genes and examined the effectiveness of PCR screening of irradiated deletion-mutant candidates at early growth stages. We believe this research is a step toward more efficient and controlled mutation breeding using ion beams.

Journal Articles

Molecular characterization of an anthocyanin-related glutathione $$S$$-transferase gene in cyclamen

Kitamura, Satoshi; Akita, Yusuke; Ishizaka, Hiroshi*; Narumi, Issei; Tanaka, Atsushi

Journal of Plant Physiology, 169(6), p.636 - 642, 2012/04

 Times Cited Count:46 Percentile:86.43(Plant Sciences)

In order to identify the anthocyanin-related GST in cyclamen, four candidates of GSTs (CkmGST1 to CkmGST4) were isolated. Phylogenetic analysis indicated that CkmGST3 was closely related to PhAN9, an anthocyanin-related GST of petunia. Expression analysis at different developmental stages of petals revealed that CkmGST3 was strongly expressed in paler pigmented petals than in fully pigmented petals, in contrast to the constitutive expression of the other three candidates during petal development. This expression pattern of CkmGST3 was correlated with those of other anthocyaninbiosynthetic genes such as CkmF3'5'H and CkmDFR2. Molecular complementation of Arabidopsis ${it tt19}$, a knockout mutant of an anthocyanin-related GST gene, demonstrated that CkmGST3 could complement the anthocyanin-less phenotype of ${it tt19}$. Transgenic plants that expressed the other three CkmGSTs did not show anthocyanin accumulation. These results indicate CkmGST3 functions in anthocyanin accumulation in cyclamen.

Journal Articles

Isolation and characterization of the fragrant cyclamen ${it O}$-methyltransferase involved in flower coloration

Akita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; et al.

Planta, 234(6), p.1127 - 1136, 2011/12

 Times Cited Count:22 Percentile:62.41(Plant Sciences)

Journal Articles

Red-purple flower due to delphinidin 3,5-diglucoside, a novel pigment for ${it Cyclamen}$ spp., generated by ion-beam irraidation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 65, 2011/01

no abstracts in English

Journal Articles

Effects of ion-beam irradiation on germination and growth of seedlings of red pepper "Hirosaki zairai"

Tomikawa, Masaru*; Maeda, Tomoo*; Honda, Kazushige*; Saga, Koichi*; Ishikawa, Ryuji*; Akita, Yusuke; Yoshihara, Ryohei; Nozawa, Shigeki; Narumi, Issei

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 73, 2011/01

Journal Articles

Comparative analysis of floral pigmentation between wild-type and white-flowered varieties of ${it Cyclamen graecum}$

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi

Journal of Horticultural Science & Biotechnology, 85(5), p.437 - 443, 2010/09

The flower colour of ${it Cyclamen graecum}$ gra6 (wild-type) is pink-purple in the flower. On the other hand, flowers of ${it C. graecum}$ gra50 (white-flowered variant) exhibit a white colour in its flower. In this study, the relationship between floral pigmentation and expression of several anthocyanin biosynthesis genes was investigated in ${it C. graecum}$ gra6 and gra50. The pigments in the flower consist mainly of malvidin 3,5-diglucoside in gra6. White-flowered ${it C. graecum}$ gra50 possessed lower amounts of anthocyanins, but higher amounts of flavonols compared to gra6. Gene expression analysis demonstrated that expression of the dihydroflavonol 4-reductase gene 2 (${it CgraDFR2}$) was lower in gra50 compared with gra6, whereas the expression of other key genes did not differ greatly. These results suggest that the white-flowered variant of gra50 may result from a defect in expression of the ${it CgraDFR2}$ gene.

Journal Articles

Red-purple flower due to delphinidin 3,5-diglucosides, a novel pigment for ${it Cyclamen}$ spp., generated by ion-beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

Plant Biotechnology, 26(5), p.565 - 569, 2009/01

 Times Cited Count:27 Percentile:65.26(Biotechnology & Applied Microbiology)

Fragrant cyclamen cultivar (${it C. persicum}$ $$times$$ ${it C. purpurascens}$), that bloomed purple flower containing malvidin 3,5-diglucoside as the major anthocyanin, was irradiated with a 320-MeV carbon-ion beam at 0-16 Gy to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants irradiated at 2 Gy were flower-color mutants that retained desirable flower shape, flower size, and leaf color. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. Because the major anthocyanins in flowers of ${it Cyclamen}$ spp. were previously restricted to malvidin, peonidin, and cyanidin types, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

Oral presentation

Measurement of laser induced residual stress of Si-Fe single-crystal using synchrotron X-ray diffraction

Sakai, Yusuke*; Akita, Koichi*; Oya, Shinichi*; Sueyoshi, Kazuyuki*; Shobu, Takahisa; Imafuku, Muneyuki*; Suzuki, Kanki*; Iwata, Keiji*

no journal, , 

no abstracts in English

Oral presentation

Comparative analysis of floral pigmentation between Cyclamen graecum and its colorless mutant

Akita, Yusuke; Ishizaka, Hiroshi*; Shimada, Akihiko; Nakayama, Masayoshi*; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Mutation induction in fragrance cyclamen (${it Cyclamen persicum}$ x ${it C. purpurascens}$) by ion beam re-irradiation

Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Comparative analysis of flavonoid biosynthesis genes between ${it Cyclamen graecum}$ and its white-flowered mutant

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

Oral presentation

Analysis of flower pigmentation genes in cyclamen

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Effects of ion-beam irradiation on germination and growth of seedlings of redpepper "Hirosakizairai"

Tomikawa, Masaru*; Maeda, Tomoo*; Honda, Kazushige*; Saga, Koichi*; Ishikawa, Ryuji*; Akita, Yusuke; Nozawa, Shigeki; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Induction of flower color mutation in fragrance cyclamen, "Kaori-no-mai" by ion beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

One of the fragrant cyclamens, Kaori-no-mai, blooms purple flowers containing malvidin 3,5-diglucoside as the major anthocyanin. Here, we irradiated etiolated petioles of Kaori-no-mai with a 320-MeV carbon-ion beam to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants were flower-color mutan. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. The major anthocyanins in flowers of ${it Cyclamen }$ spp. were previously restricted to malvidin, peonidin, and cyanidin types, therefore, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

Oral presentation

Mutation induction in fragrance cyclamen "Uruwashi-no-kaori" by ion beam irradiation

Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Kurihara, Yasushi*; Akita, Yusuke; Tanikawa, Natsu*; Morita, Yasumasa*; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of red-purple mutant generated by ion beam irradiation from haploid of fragrant purple cyclamen "Kaori-no-mai"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kameari, Naoko*; Nakayama, Masayoshi*; Kondo, Emiko*; Kurihara, Yasushi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of red-purple mutants generated by ion beam irradiation from fragrant purple cyclamen "Kaori-no-mai"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of white mutants generated by ion beam irradiation from fragrant purple cyclamen "Kokou-no-kaori"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

41 (Records 1-20 displayed on this page)