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Dong, M. A.*; Bufford, J. L.*; Ono, Yutaka; Church, K.*; Dau, M. Q.*; Michels, K.*; Haughton, M.*; Tallman, G.*
Plant Physiology, 145(2), p.367 - 377, 2007/10
Times Cited Count:3 Percentile:9.32(Plant Sciences)Cultured guard cell protoplasts (GCP) of Nicotiana glauca, tree tobacco, comprise a novel system for investigating the cell signaling mechanisms that lead to acquired thermotolerance and thermoinhibition. At 32 C in a medium containing an auxin and a cytokinin, GCP expand, regenerate cell walls, dedifferentiate, and divide. At 38 C, GCP acquire thermotolerance, but their expansion is limited and they neither regenerate walls nor re-enter the cell cycle. Protoplasts were transformed with the fusion gene of BA auxin-responsive promoter and green fluorescent protein (GFP) gene. Heat suppressed auxin-mediated activation of BA. Heat-stressed cells accumulate reactive oxygen species, and HO suppresses auxin-responsive promoter activation. HO did not suppress BA activation at 32 C, nor did superoxide and HO scavengers prevent BA suppression at 38 C.
Dong, M.*; Bufford, J. L.*; Ono, Yutaka; Church, K.*; Dau, M. Q.*; Michels, K.*; Haughton, M.*; Tallman, G.*
no journal, ,
At 32C in media containing an auxin and a cytokinin, cultured guard cell protoplasts (GCP) of Nicotiana glauca (Graham), expand, regenerate cell walls, dedifferentiate, and divide. At 38C, GCP acquire thermotolerance within 24 h but their expansion is limited and they neither regenerate walls nor re-enter the cell cycle. Protoplasts were transformed with BA-mgfp5-ER. After 21 h in media with NAA, 49.0% and 7.9% of GCP transformed with BA-mgfp5-ER expressed GFP at 32C and 38C, respectively. Expression at 38C was not increased by incubating GCP for 48 h or by increasing NAA concentrations 20X. Tiron and N-acetylcysteine, superoxide and HO scavengers, respectively, did not prevent BA suppression at 38C nor did H O suppress BA activation at 32C.