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R1Jolivet, E.*; Lecointe, F.*; Coste, G.*; Sato, Katsuya*; Narumi, Issei; Bailone, A.*; Sommer, S.*
Molecular Microbiology, 59(1), p.338 - 349, 2006/01
Times Cited Count：40 Percentile：57.25(Biochemistry & Molecular Biology)To evaluate the importance of RecA in DNA double strand break (DSB) repair, we examined the effect of low and high RecA concentration such as 2,500 and 100,000 molecules per cell from the inducible P
promoter in in absence or in presence of IPTG, respectively. We showed that at low concentration, RecA has a negligible effect on cell survival after 
-irradiation when bacteria were immediately plated on TGY agar whereas it significantly decreased the survival to -irradiation of 
cells while overexpression of RecA can partially compensate the loss of DdrA protein. In contrast, when cells expressing limited concentration of RecA were allowed to recover in TGY liquid medium, they showed a delay in mending DSB, failed to reinitiate DNA replication and were committed to die during incubation in liquid medium. A deletion of 
resulted in sensitivity to -irradiation and mitomycin C treatment. Interestingly, constitutive high expression of RecA compensates partially the sensitization to mitomycin C. The cells with low RecA content also failed to cleave LexA after DNA damage. However, neither a deletion of the 
gene nor the expression of a non cleavable LexA(Ind
) mutant protein, had an effect on survival or kinetics of DNA DSB repair compared to their 
counterparts in 
as well as in bacteria expressing limiting concentration of RecA, suggesting an absence of relationship between the loss of viability, the delay in the kinetic of DSB repair, and the absence of LexA cleavage. Thus, LexA protein seems to play no major role in the recovery processes after -irradiation in 
.
Yamano, Hidemasa; Fujita, Satoshi; Tobita, Yoshiharu; Kamiyama, Kenji; Kondo, Satoru; Morita, Koji*; Fischer, E. A.; Brear, D. J.; Shirakawa, Noriyuki*; Cao, X.; et al.
JNC TN9400 2003-071, 340 Pages, 2003/08
JNC-TN9400-2003-071.pdf:1.54MB
An advanced safety analysis computer code, SIMMER-III, has been developed to investigate postulated core disruptive accidents in liquid-metal fast reactors (LMFRs). SIMMER-III is a two-dimensional, three-velocity-field, multiphase, multicomponent, Eulerian, fluid-dynamics code coupled with a space-dependent neutron kinetics model. By completing and integrating all the physical models originally intended at the beginning of this code development project, SIMMER-III is now applicable to integral reactor calculations and other complex multiphase flow problems. A systematic code assessment program, conducted in collaboration with European research organizations, has shown that the advanced features of the code have resolved many of the limitations and problem areas in the previous SIMMER-II code. In this report, the models, numerical algorithms and code features of SIMMER-III Version 3.A are described along with detailed program description. Areas which require future model refinement are also discussed. SIMMER-III Version 3.A, a coupled fluid-dynamics and neutronics code system, is expected to significantly improve the flexibility and reliability of LMFR safety analyses.
