JOPSS - Search Results

Search Results: Records 1-4 displayed on this page of 4

Presentation/Publication Type

Initialising ...

Refine

Journal/Book Title

Initialising ...

Meeting title

Initialising ...

First Author

Initialising ...

Keyword

Initialising ...

Language

Initialising ...

Publication Year

Initialising ...

Held year of conference

Initialising ...

Journal Articles

Induction of DNA strand breaks, base lesions and clustered damage sites in hydrated plasmid DNA films by ultrasoft X rays around the phosphorus K edge

Yokoya, Akinari; Cunniffe, S. M. T.*; Watanabe, Ritsuko; Kobayashi, Katsumi*; O'Neill, P.*

Radiation Research, 172(3), p.296 - 305, 2009/09

https://doi.org/10.1667/RR1609.1

Times Cited Count：25 Percentile：61.06(Biology)

To characterize the DNA damage induced by K-shell ionization of phosphorus atom in DNA backbone on the level of hydration, the yields of DNA strand breaks and base lesions arising from the interaction of ultrasoft X-rays (USX) with energies around the phosphorus K-edge were determined using dry and fully hydrated pUC18 plasmid DNA samples. Base lesions and bistranded clustered DNA damage sites were revealed by post-irradiation treatment with the base excision repair proteins, Nth and Fpg. The complex types of damage, prompt and enzymatically induced DSB are preferentially induced by phosphorus K-resonance at 2153 eV rather than simple SSB and isolated base lesions particularly in hydrated conditions. It is concluded that not only the phosphorus K-resonance and resulting emission of low energy LMM-Auger electrons (120 eV) but also the level of hydration plays an important role in the induction of complex damage in plasmid DNA.