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ray-induced mutagenesisSaito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Ono, Yutaka; Narumi, Issey*
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 121, 2015/03
(Hypocreales: Clavicipitaceae) mutants induced by ion beamsFitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*
Applied Entomology and Zoology, 50(1), p.123 - 129, 2015/02
Times Cited Count：0 Percentile：1.75(Chemistry, Physical)-ray irradiations induce thermotolerant mutants in the entomopathogenic fungus 
Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*
Biocontrol Science and Technology, 24(9), p.1052 - 1061, 2014/06
Times Cited Count：3 Percentile：16.13(Biotechnology & Applied Microbiology)Saito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*
JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 116, 2014/03
following ionizing radiation-induced mutagenesisShinohara, Shinobu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*
FEMS Microbiology Letters, 349(1), p.54 - 60, 2013/12
Times Cited Count：11 Percentile：29.5(Microbiology)Fitriana, Y.*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
Thermotolerant mutants of entomopathogenic fungi, , and 
, were screened from conidia irradiated with carbon ion beams at doses ranged from 100 to 1000 Gy. Sabouraud dextrose broth (SDB) and Sabouraud dextrose agar (SDA) were used for the screening. The wild-type strain of the three fungal species formed colonies with abnormal shape on SDA at 33
C. For 
, 41 isolates that could normally grow in SDB at 35C were successfully obtained, and finally 5 isolates were selected as potential mutants that will be used for further studies. Any normal colonies could not be obtained from SDA at 35C. The results show that ion beam irradiation is useful to generate thermotolerant mutants of entomopathogenic fungi. However, thermotolerant mutants of 
and 
have not been obtained yet.
Saito, Tsutomu*; Fitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*
no journal, ,
In order to identify the mutation sites of 
-tubulin in the benomyl-tolerant mutants, we determined the nucleotide sequences of -tubulin locus and compared with those of the wild-type strains. In , the -tubulin sequences were identical to that of the wild-type, suggesting that other mechanisms might be responsible for the benomyl tolerance. On the other hand, in , the mutation was identified to be an A:T to C:G transversion at position 924 in the -tubulin gene. This mutation causes an amino acid substitution at position 198 (Glu to Ala) in the -tubulin protein. This site might be a binding target of benomyl or adjacent to the center of benomyl interaction with the -tubulin. These results suggest that mechanisms of benomyl tolerance were different between and .
Saito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*
no journal, ,
no abstracts in English
