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Journal Articles

Thermotolerant mutants of entomopathogenic fungi obtained by ion beam- and $$gamma$$ ray-induced mutagenesis

Saito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Ono, Yutaka; Narumi, Issey*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 121, 2015/03

Journal Articles

Benomyl-resistant ${it Beauveria bassiana}$ (Hypocreales: Clavicipitaceae) mutants induced by ion beams

Fitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*

Applied Entomology and Zoology, 50(1), p.123 - 129, 2015/02

 Times Cited Count:0 Percentile:100(Chemistry, Physical)

Journal Articles

Ion-beam and $$gamma$$-ray irradiations induce thermotolerant mutants in the entomopathogenic fungus ${it Metarhizium anisopliae s.l.}$

Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*

Biocontrol Science and Technology, 24(9), p.1052 - 1061, 2014/06

 Times Cited Count:2 Percentile:82.01(Biotechnology & Applied Microbiology)

Journal Articles

Benomyl-tolerant mutation of entomopathogenic fungi induced by carbon ion beams

Saito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 116, 2014/03

Journal Articles

Enhanced fungicide resistance in ${it Isaria fumosorosea}$ following ionizing radiation-induced mutagenesis

Shinohara, Shinobu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*

FEMS Microbiology Letters, 349(1), p.54 - 60, 2013/12

 Times Cited Count:9 Percentile:65.64(Microbiology)

Oral presentation

Development of thermotolerant mutants of entomopathogenic fungi using ion beam irradiation

Fitriana, Y.*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*

no journal, , 

Thermotolerant mutants of entomopathogenic fungi, ${it Beauveria bassiana}$, ${it Isaria fumosorosea}$ and ${it Metarhizium anisopliae}$, were screened from conidia irradiated with carbon ion beams at doses ranged from 100 to 1000 Gy. Sabouraud dextrose broth (SDB) and Sabouraud dextrose agar (SDA) were used for the screening. The wild-type strain of the three fungal species formed colonies with abnormal shape on SDA at 33$$^{circ}$$C. For ${it M. anisopliae}$, 41 isolates that could normally grow in SDB at 35$$^{circ}$$C were successfully obtained, and finally 5 isolates were selected as potential mutants that will be used for further studies. Any normal colonies could not be obtained from SDA at 35$$^{circ}$$C. The results show that ion beam irradiation is useful to generate thermotolerant mutants of entomopathogenic fungi. However, thermotolerant mutants of ${it B. bassiana}$ and ${it I. fumosorosea}$ have not been obtained yet.

Oral presentation

Benomyl-tolerant mutation of entomopathogenic fungi induced by carbon ion beams

Saito, Tsutomu*; Fitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*

no journal, , 

In order to identify the mutation sites of $$beta$$-tubulin in the benomyl-tolerant mutants, we determined the nucleotide sequences of $$beta$$-tubulin locus and compared with those of the wild-type strains. In ${it Isaria fumosorosea}$, the $$beta$$-tubulin sequences were identical to that of the wild-type, suggesting that other mechanisms might be responsible for the benomyl tolerance. On the other hand, in ${it Beauveria bassiana}$, the mutation was identified to be an A:T to C:G transversion at position 924 in the $$beta$$-tubulin gene. This mutation causes an amino acid substitution at position 198 (Glu to Ala) in the $$beta$$-tubulin protein. This site might be a binding target of benomyl or adjacent to the center of benomyl interaction with the $$beta$$-tubulin. These results suggest that mechanisms of benomyl tolerance were different between ${it I. fumosorosea}$ and ${it B. bassiana}$.

Oral presentation

Thermotolerant mutants of entomopathogenic fungi obtained by quantum beam-induced mutagenesis

Saito, Tsutomu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*

no journal, , 

no abstracts in English

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