Visualising spatio-temporal distributions of assimilated carbon translocation and release in root systems of leguminous plants

Yin, Y.-G.*; Suzui, Nobuo*; Kurita, Keisuke; Miyoshi, Yuta*; Unno, Yusuke*; Fujimaki, Shu*; Nakamura, Takuji*; Shinano, Takuro*; Kawachi, Naoki*

Scientific Reports (Internet), 10, p.8446_1 - 8446_11, 2020/06

https://doi.org/10.1038/s41598-020-65668-9

The release of rhizodeposits differs depending on the root position and is closely related to the assimilated carbon (C) supply. Therefore, quantifying the C partitioning over a short period may provide crucial information for clarifying root-soil carbon metabolism. A non-invasive method for visualising the translocation of recently assimilated C into the root system inside the rhizobox was established using CO labelling and the positron-emitting tracer imaging system. The spatial distribution of recent C-photoassimilates translocated and released in the root system and soil were visualised for white lupin () and soybean (). Our method enabled the quantification of the spatial C allocations in roots and soil, which may help to elucidate the relationship between C metabolism and nutrient cycling at specific locations of the root-soil system in response to environmental conditions over relatively short periods.

Non-invasive imaging of sodium dynamics in common reed using positron-emitting tracer imaging system

Suzui, Nobuo; Maruyama, Teppei*; Kawachi, Naoki; Miwa, Eitaro*; Higuchi, Kyoko*; Fujimaki, Shu

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 95, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Development of a gamma camera system for high-energy gamma photon for quantitative observation of Cs movement in a plant body

Kawachi, Naoki; Yin, Y.-G.; Suzui, Nobuo; Ishii, Satomi; Yoshihara, Toshihiro*; Watabe, Hiroshi*; Yamamoto, Seiichi*; Fujimaki, Shu

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 94, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Imaging of radiocesium uptake dynamics in a plant body by using a newly developed high-resolution gamma camera

Kawachi, Naoki; Yin, Y.-G.; Suzui, Nobuo; Ishii, Satomi; Yoshihara, Toshihiro*; Watabe, Hiroshi*; Yamamoto, Seiichi*; Fujimaki, Shu

Journal of Environmental Radioactivity, 151(Part 2), p.461 - 467, 2016/01

https://doi.org/10.1016/j.jenvrad.2015.04.009

Evidence for Na exclusion to the direction of root tips in common reed

Higuchi, Kyoko*; Fujimaki, Shu

Bio Industry, 32(12), p.46 - 52, 2015/12

no abstracts in English

Analysis of movement of radiocesium in a plant body using a gamma-camera

Fujimaki, Shu

Hoshasen To Sangyo, (138), p.25 - 28, 2015/06

no abstracts in English

Base to tip and long-distance transport of sodium in the root of common reed [ (Cav.) Trin. ex Steud.] at steady state under constant high-salt conditions

Fujimaki, Shu; Maruyama, Teppei*; Suzui, Nobuo; Kawachi, Naoki; Miwa, Eitaro*; Higuchi, Kyoko*

Plant & Cell Physiology, 56(5), p.943 - 950, 2015/05

https://doi.org/10.1093/pcp/pcv021

We analyzed the directions and rates of translocation of sodium ions (Na) within tissues of a salt-tolerant plant, common reed, and a salt-sensitive plant, rice, under constant high-salt conditions using radioactive Na tracer and a positron-emitting tracer imaging system (PETIS). First, the test plants were incubated in a nutrient solution containing 50 mM NaCl and a trace level of Na for 24 h (feeding step). Then the original solution was replaced with a fresh solution containing 50 mM NaCl but no Na, in which the test plants remained for over 48 h (chase step). Non-invasive visualization of Na movement in the test plants was conducted during feeding and chase steps with PETIS. Our results revealed that Na was absorbed in the roots of common reed, but not transported to the upper shoot beyond the shoot base. During the chase step, a basal-to-distal movement of Na was detected within the root tissue with a velocity of approximately 0.5 cm h. On the other hand, Na that absorbed in the roots of rice was continuously translocated to the whole shoot. We concluded that the basal roots and the shoot base of common reed have constitutive functions of Na exclusion only in the direction of root tips, even under constant high-salt conditions. This function may apparently contribute to the low Na concentration in the upper shoot and high salt tolerance of common reed.

Live-imaging evaluation of the efficacy of elevated CO concentration in a closed cultivation system for the improvement of bioproduction in tomato fruits

Yamazaki, Haruaki*; Suzui, Nobuo; Yin, Y.-G.; Kawachi, Naoki; Ishii, Satomi; Shimada, Hiroaki*; Fujimaki, Shu

Plant Biotechnology, 32(1), p.31 - 37, 2015/04

https://doi.org/10.5511/plantbiotechnology.14.1210a

Evaluation of velocity of C-photoassimilate flow using positron-emitting tracer imaging system

Suzui, Nobuo; Kawachi, Naoki; Ishii, Satomi; Yin, Y.-G.; Iwasaki, Aya*; Ogawa, Kenichi*; Fujimaki, Shu

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 105, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=137

Effects of glutathione concentration in the root zone and glutathione treatment period on cadmium partitioning in oilseed rape plants

Nakamura, Shinichi*; Kondo, Hikari*; Suzui, Nobuo; Yin, Y.-G.; Ishii, Satomi; Kawachi, Naoki; Rai, Hiroki*; Hattori, Hiroyuki*; Fujimaki, Shu

Molecular Physiology and Ecophysiology of Sulfur, p.253 - 259, 2015/00

https://doi.org/10.1007/978-3-319-20137-5_28