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藤浪 俊*; 武田 喜代子*; 小野寺 威文*; 佐藤 勝也; 清水 哲*; 若林 佑*; 鳴海 一成*; 中村 顕*; 伊藤 政博*
Genome Announcements (Internet), 3(5), p.e01005-15_1 - e01005-15_2, 2015/09
sp. ME121 was isolated from soil as a mixed single colony with sp. 32K during our screening of L-glucose-utilizing microorganisms, and its growth was enhanced by coculture. It was expected that genomic analysis of this bacterium would provide novel information on coculture-dependent growth enhancement. The genomic information of symbiotic bacteria could be of use for studying the molecular mechanisms underlying microbial symbiosis. The draft genome sequence of sp. ME121 is 7,096,979 bp in total length and comprises 197 large contigs ( 500 bp) that was deposited at DDBJ/EMBL/GenBank under the accession number BBUX00000000. The draft genome sequence shows that sp. ME121 has some genes that encode putative methanol/ethanol family PQQ-dependent dehydrogenases involved in methylotrophy. Some unknown factor provided by the coculture may contribute to increase the growth of sp. ME121.
藤浪 俊*; 武田 喜代子*; 小野寺 威文*; 佐藤 勝也; 佐野 元彦*; 高橋 優嘉*; 鳴海 一成*; 伊藤 政博*
Genome Announcements (Internet), 2(5), p.e00866-14_1 - e00866-14_2, 2014/09
Calcium-dependent sp. strain TCA20 was isolated from a water sample of a hot spring containing a high concentration of calcium ions. Here, we report the draft genome sequence of this bacterium, which may be the basis for the research of calcium ion homeostasis. The draft genome sequence of sp. strain TCA20 totals 5,631,463 bp in length and is composed of 33 large contigs ( 500 bp) that was deposited at DDBJ/EMBL/Genbank under the accession no. BBIW00000000. The annotation of the draft genome sequence shows that sp. strain TCA20 has a calcium-specific calcium/proton antiporter, ChaA, and a P-type calcium-transporting ATPase, YloB, was identified as calcium transporters. These transporters may also be important for the growth of sp. strain TCA20 under the high concentration of calcium ions.
藤浪 俊*; 武田 喜代子*; 小野寺 威文*; 佐藤 勝也; 佐野 元彦*; 鳴海 一成*; 伊藤 政博*
Genome Announcements (Internet), 2(3), p.e00458-14_1 - e00458-14_2, 2014/05
The potassium-dependent alkaliphilic sp. strain TS-2 was isolated from the mashed extract of a jumping spider, and its draft genome sequence was obtained. Comparative genomic analysis with a previously sequenced sodium-dependent alkaliphilic species may reveal potassium-dependent alkaline adaptation mechanisms. The draft genome sequence of sp. strain TS-2 was 4,360,646 bp in total length, comprised 58 large contigs ( 500 bp) that was deposited at DDBJ/EMBL/Genbank under the accession number BAWL00000000. In the draft genome sequence of sp. strain TS-2, two sets of genes, which encode multisubunit secondary cation/proton antiporter-3 family proteins, were annotated. The Mrp complex acts as an Na/H antiporter in typical alkaliphilic species and plays a critical role in the sodium-dependent alkaline adaptation mechanism.
藤浪 俊*; 武田 喜代子; 小野寺 威文*; 佐藤 勝也; 佐野 元彦*; 鳴海 一成*; 伊藤 政博*
Genome Announcements (Internet), 1(6), P. e01043-13, 2013/12
Alkaliphilic sp. strain TS-1, newly isolated from the jumping spider, showed Na-independent growth and motility. Here, we report the draft genome sequence of this bacterium, which may provide beneficial information for Na-independent alkaline adaptation mechanisms. The draft genome sequence of sp. strain TS-1 was 3,396,165 bp in total length and comprised 3 large contigs (2,699,404 bp, 691,278 bp, and 5,483 bp) that was deposited at DDBJ/ EMBL/GenBank under the accession number BASQ00000000. The annotation of the draft genome sequence indicated that this bacterium has 3 sets of genes, which encode multisubunit secondary cation/proton antiporter-3 family proteins for pH homeostasis. The annotation also indicated that this bacterium has genes involved in chemotaxis/flagellar components, including one set of genes.
今澤 陸*; 藤浪 俊*; 高橋 優嘉*; 佐藤 勝也; 鳴海 一成*; 伊藤 政博*
no journal, ,
細胞の生育に大きく関与するCaは、細胞内でその濃度が厳密に調節されている。しかし、原核生物の細胞内Ca濃度の調節機構については、未だ不明な点が多い。本研究では、カルシウム含有量が高い弱アルカリ性温泉地から分離されたCaを生育に要求する sp. TCA20株を用いて、細胞内外のCaサイクルの詳細を理解するために、ゲノム解析を行った。その結果、Ca/Hアンチポーターをコードする遺伝子およびP型Ca-ATPaseをコードする遺伝子のホモログを持つことから、これらの遺伝子群がCaサイクルや生育に重要である可能性が示唆された。