Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams

高野 成央*; 高橋 祐子*; 山本 充*; 寺西 美佳*; 山口 弘子*; 坂本 綾子; 長谷 純宏; 藤澤 弘子*; Wu, J.*; 松本 隆*; et al.

Journal of Radiation Research, 54(4), p.637 - 648, 2013/07

https://doi.org/10.1093/jrr/rrt007

UVB radiation suppresses photosynthesis and protein biosynthesis in plants, which in turn decreases growth and productivity. Here, an ultraviolet-B (UVB)-tolerant rice mutant, (), was isolated from a mutagenized population derived from 2,500 M1 seeds that were exposed to carbon ions. The mutant was more tolerant to UVB than the wild type. Neither the levels of UVB-induced CPDs, nor the repair of cyclobutane pyrimidine dimers or (6-4) pyrimidine-pyrimidone photodimers was altered in the mutant. Thus, the mutant may be impaired in the production of a previously unidentified factor that confers UVB tolerance. To identify the mutated region in the mutant, microarray-based comparative genomic hybridization analysis was performed. Two adjacent genes on chromosome 7were predicted to represent the mutant allele. Sequence analysis of the chromosome region in revealed a deletion of 45,419 bp. Database analysis indicated that the Os07g0265100 gene, , encodes a putative protein with unknown characteristics or function. In addition, the homologs of UTR319 are conserved only among land plants. Therefore, is a novel UVB-tolerant rice mutant and UTR319 may be crucial for the determination of UVB sensitivity in rice, although the function of UTR319 has not yet been determined.