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Sakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Shimozawa, Yoko; Fukamoto, Kana*; Yokota, Yuichiro; Sora, Sakura*; Kakizaki, Takehiko*; Wada, Seiichi*; Funayama, Tomoo; et al.
Biological Sciences in Space, 26, p.21 - 25, 2012/10
High linear energy transfer (LET) radiation is important cosmic rays that has neurobiological effects: it is known to induce conditioned taste aversion, and suppress neurogenesis that may underlie cognitive impairment. However, the impact of high-LET radiation on other learning effects remains largely unknown. Here, we focus on kinetics of the radiation response for the salt chemotaxis learning (SCL) behavior in the nameatode, , because the SCL during the learning conditioning was modulated after low-LET 
-irradiation. Firstly, the SCL ability was examined following high-LET irradiation (
C, 18.3 MeV/u, LET = 113 keV/
m), revealing its dose-dependent decrease after high- and low-LET exposure. Next, we demonstrate that the SCL at the early phase of the learning conditioning is greatly affected by high- and low-LET irradiation, and interestingly, the magnitude of these effects by high-LET radiation was smaller than that by low-LET one. Moreover, the analysis of 
mutant showed that the G-protein subunit, GPC-1 is responsible for such early phase response. This study is the first to provide the evidence for the kinetics of changes in SCL after high-LET irradiation of C. 
.
against high-LET radiation exposureSakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Shimozawa, Yoko; Fukamoto, Kana*; Yokota, Yuichiro; Sora, Sakura*; Kakizaki, Takehiko*; Wada, Seiichi*; Funayama, Tomoo; et al.
Biological Sciences in Space, 26, p.7 - 11, 2012/07
Here, we investigated the resistance to high-LET radiation exposure for two behaviors of the nematode, , which is known as a model organism for the nervous system. Tested behaviors were locomotion and chemotaxis to NaCl. In addition, egg hatchability was examined as an indicator of high-LET radiation sensitivity. Relative biological effectiveness (RBE) of high-LET radiation (C, 18.3 MeV/u, LET = 113 keV/m) relative to low-LET radiation for hatchability was 4.5, whereas RBEs for locomotion and chemotaxis were 1.4 and 1.1, respectively. This study shows that the behavioral system for locomotion and chemotaxis of 
is highly resistant to high-LET radiation exposure.
Hino, Mizuki*; Hamada, Nobuyuki*; Tajika, Yuki*; Funayama, Tomoo; Morimura, Yoshihiro*; Sakashita, Tetsuya; Yokota, Yuichiro; Fukamoto, Kana*; Muto, Yasuko; Kobayashi, Yasuhiko; et al.
Journal of Electron Microscopy, 59(6), p.495 - 501, 2010/12
Times Cited Count：16 Percentile：63.58(Microscopy)
, induces the somatic mutationFurusawa, Toshiharu*; Fukamoto, Kana*; Sakashita, Tetsuya; Suzuki, Eiko*; Kakizaki, Takehiko*; Hamada, Nobuyuki*; Funayama, Tomoo; Suzuki, Hiromi*; Ishioka, Noriaki*; Wada, Seiichi*; et al.
Journal of Radiation Research, 50(4), p.371 - 375, 2009/07
Times Cited Count：9 Percentile：31.53(Biology)Using heavy-ion microbeam, we report target irradiation of selected compartments within the diapause-terminated egg and its mutational consequences in the silkworm, . On one hand, carbon-ion exposure of embryo to 0.5 - 6 Gy increased the somatic mutation frequency, suggesting targeted radiation effects. On the other, such increases were not observed when yolk was targeted, suggesting a lack of nontargeted bystander effect.
Suzuki, Michiyo; Sakashita, Tetsuya; Yanase, Sumino*; Kikuchi, Masahiro; Oba, Hirofumi; Higashitani, Atsushi*; Hamada, Nobuyuki*; Funayama, Tomoo; Fukamoto, Kana; Tsuji, Toshio*; et al.
Journal of Radiation Research, 50(2), p.119 - 125, 2009/04
Times Cited Count：8 Percentile：29.13(Biology)Hino, Mizuki*; Hamada, Nobuyuki*; Tajika, Yuki*; Funayama, Tomoo; Morimura, Yoshihiro*; Sakashita, Tetsuya; Yokota, Yuichiro; Fukamoto, Kana*; Kobayashi, Yasuhiko; Yorifuji, Hiroshi*
Cell Structure and Function, 34(1), p.11 - 15, 2009/03
Times Cited Count：12 Percentile：20.47(Cell Biology)Kobayashi, Yasuhiko; Funayama, Tomoo; Hamada, Nobuyuki*; Sakashita, Tetsuya; Yokota, Yuichiro; Fukamoto, Kana; Suzuki, Michiyo; Taguchi, Mitsumasa
Hoshasen Seibutsu Kenkyu, 43(2), p.150 - 169, 2008/06
no abstracts in English
, as a simple model for studing wound healingFukamoto, Kana
Sanshi, Konchu Biotekku, 77(1), p.17 - 18, 2008/04
no abstracts in English
Furusawa, Toshiharu*; Suzuki, Eiko*; Nagaoka, Shunji*; Suzuki, Hiromi*; Ishioka, Noriaki*; Hamada, Nobuyuki*; Wada, Seiichi*; Kobayashi, Yasuhiko; Sakashita, Tetsuya; Kakizaki, Takehiko*; et al.
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 115, 2008/03
Using heavy ion microbeam, we investigated the somatic mutation arising on the larvae in embyro and yolk in the egg of silkworm, . The incidence of the somatic mutation was 12%, and the same level of mutation following the microbeam irradiation at the center of the egg. However, the microbeam irradiation to the abdomen of the silkworm larvae induced the increase of somatic mutation, 63% (3 Gy) and 80% (6 Gy).
to -ray irradiationSakashita, Tetsuya; Suzuki, Michiyo; Fukamoto, Kana; Funayama, Tomoo; Wada, Seiichi*; Kobayashi, Yasuhiko; Horikawa, Daiki*; Bolige, A.*
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 110, 2008/03
We investigated the effects of rays on the salt chemotaxis learning in . We observed no decrease of performance of the salt chemotaxis learning following 500 Gy irradiation. Also, showed the normal chemotaxis to benzaldehyde, whereas the salt chemotaxis learning were affected by irradiation during learning.
Funayama, Tomoo; Wada, Seiichi*; Yokota, Yuichiro; Fukamoto, Kana; Sakashita, Tetsuya; Taguchi, Mitsumasa; Kakizaki, Takehiko*; Hamada, Nobuyuki*; Suzuki, Michiyo; Furusawa, Yoshiya*; et al.
Journal of Radiation Research, 49(1), p.71 - 82, 2008/01
Times Cited Count：47 Percentile：78.15(Biology)Research concerning cellular responses to low dose irradiation, radiation-induced bystander effects, and the biological track structure of charged particles has recently received particular attention in the field of radiation biology. Target irradiation employing a microbeam represents a useful means of advancing this research by obviating some of the disadvantages associated with the conventional irradiation strategies. The heavy-ion microbeam system at JAEA-Takasaki can provide target irradiation of heavy charged particles to biological material at atmospheric pressure using a minimum beam size 5 m in diameter. The system can be applied to the investigation of mechanisms within biological organisms not only in the context of radiation biology, but also in the fields of general biology such as physiology, developmental biology and neurobiology, and should help to establish and contribute to the field of "microbeam biology".
Fukamoto, Kana; Shirai, Koji*; Sakata, Toshiyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kakizaki, Takehiko; Shimura, Sachiko*; Kobayashi, Yasuhiko; et al.
Journal of Radiation Research, 48(3), p.247 - 253, 2007/05
Times Cited Count：17 Percentile：47.23(Biology)To carry out the radio-microsurgery study using silkworm, , we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-mum-diameter microbeam of 220 MeV carbon (C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6 % of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.
Kobayashi, Yasuhiko; Funayama, Tomoo; Sakashita, Tetsuya; Furusawa, Yoshiya*; Wada, Seiichi*; Yokota, Yuichiro; Kakizaki, Takehiko; Hamada, Nobuyuki*; Hara, Takamitsu*; Fukamoto, Kana; et al.
JAEA-Conf 2007-002, p.28 - 35, 2007/02
no abstracts in English
, after heavy-ion irradiation; Analysis by transplantation of the irradiated organs using a transgenic silkworm strainKiguchi, Kenji*; Shirai, Koji*; Sakata, Toshiyuki*; Fukamoto, Kana; Kakizaki, Takehiko; Wada, Seiichi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 117, 2007/02
no abstracts in English
Fukamoto, Kana; Shimura, Sachiko*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko
Journal of Insect Biotechnology and Sericology, 75(3), p.107 - 114, 2006/10
no abstracts in English
cyst formation from the implanted larval integument in the sweet potato hornworm, 
; A Simple model for studying wound healingFukamoto, Kana; Shirai, Koji*; Sato, Shigeru*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko
Journal of Insect Biotechnology and Sericology, 75(3), p.99 - 106, 2006/10
no abstracts in English
, after locally targeted irradiation with heavy ion beamsLing, E.*; Fukamoto, Kana*; Xu, S.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*
Journal of Insect Biotechnology and Sericology, 72(2), p.95 - 100, 2003/09
no abstracts in English
Sora, Sakura; Hamada, Nobuyuki*; Hara, Takamitsu*; Funayama, Tomoo; Sakashita, Tetsuya; Kataoka, Keiko*; Suzuki, Michiyo; Fukamoto, Kana; Yokota, Yuichiro; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
rays on the cell line SS4 of a silkworm, 
Fukamoto, Kana; Tsuchiya, Shiori*; Shirai, Koji*; Kiguchi, Kenji*; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Imanishi, Shigeo*
no journal, ,
no abstracts in English
; Is there any change on networks by exposure to ionizing radiation?Suzuki, Michiyo*; Sakashita, Tetsuya; Tsuji, Toshio*; Fukamoto, Kana; Hamada, Nobuyuki*; Kobayashi, Yasuhiko
no journal, ,
We have revealed the radiation-induced change on food-NaCl associative learning of without no change of chemotaxis towards benzaldehyde. Soluble and volatile chemicals are perceived by separate sensory neurons that respectively connect with multiple interneurons and motoneurons. However, since several interneurons connect with these sensory neurons, neuronal networks with respect to chemotaxis of NaCl and benzaldehyde are considerably overlapped. Therefore, although it is indicated that the change by irradiation is induced on some parts of the neuronal network of NaCl chemotaxis, the change has never been clarified. Then, we here modeled the neuronal network of chemotaxis based on actual connections to find certain parts that are induced to change by -irradiation. Through the computer simulation of the responses before and after irradiation, we tried to predict the change on the neuronal network.
